Project name: High-throughput RNA sequencing of transcriptomes expressed in Oral squamous cell carcinoma (OSCC) and human oral keratinocytes (HOK)

Description: Next-generation sequencing (NGS) has transformed systems-based analysis in the molecular landscapes of various cancer types. The goals of this study are to obtain transcriptomes expressed in human oral squamous cell carcinoma (OSCC) cell lines. The transcriptome profiles of OSCC cell lines (H357, KB and Hep-2) and normal Human oral keratinocytes (HOK) were obtained from the high throughput RNA sequencing sequencing using Illumina Hiseq2500 platform. We obtained 10160, 10251, 10191, 10201 transcripts expressed in HOK, H357, KB, and Hep-2 respectively, which include protein coding genes (PCGs), lncRNAs, pseudogenes and others. Our results showed a set of transcripts that are dysregulated in OSCC, which might be playing some key roles in tumorigenesis process. Further in-depth analysis might provide clues for better understanding of gene function in OSCC.Overall design: 1 µg of total RNA obtained from above all cell lines were used as the starting material for library preparation as prescribed by Illumina Hiseq transcriptome library protocol. The mRNA libraries were sequenced on Illumina HiSeq2500 platform. Upon de-multiplexing, reads are obtained and then analyzed after QC.

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: Medical

Last updated: 2022-02-14