Project name: Parathyroid Hormone Treatment Effect on Gene Expression in Primary Calvarial Osteoblasts

Description: The effect of parathyroid hormone (PTH) treatment was tested on osteoblasts prepared from calvaria of C57BL/6J mice 7-10 days old. Osteoblasts were treated with either human parathyroid hormone (1-34), bovine (D-Trp12,Tyr34) parathyroid hormone (7-34) amide or vehicle control for 4 hr. Gene expression effects were evaluated by DNA microarray.Overall design: Mouse osteoblasts were prepared independently for each biological replicate. Three biological replicates were used for each treatment. Osteoblasts were treated with either 100 nM human parathyroid hormone (1-34), 1 µM bovine (D-Trp12,Tyr34) parathyroid hormone (7-34) amide or vehicle for 4 hr. Total RNAs were collected and 100 ng analyzed for gene expression using Affymetrix Mouse Gene 2.0 ST GeneChips.

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: ModelOrganism

Last updated: 2021-08-01