Project name: Bulk RNA-seq profiling of murine endothelial cells in response to pulmonary hypertension

Description: Endothelial cell dysfunction plays a critical role in the development and pathogenesis of pulmonary arterial hypertension (PAH). We aimed to characterize the endothelial cell transcriptomic changes in PAH. We carried out bulk RNA sequencing of lung endothelial cells isolated from an endothelial cell lineage tracing mouse model in control and SU5416/Hypoxia-induced PAH conditions.Overall design: To study ECs in murine lungs, we used a Cdh5-CreERT2-TdTomato mouse line, in which the EC-specific (Cdh5) expression of TdTomato is inducible with tamoxifen. After a two week wash out period, we exposed the mice to chronic hypoxia for three weeks in combination with weekly subcutaneous injections of SU5416 to induce PAH while Control mice were kept in normoxic condition. RNA extraction and bulk RNA-seq were carried out on 5 Control (b-Cont1-5) and 4 PAH (b-PAH1-4) mice.

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: ModelOrganism

Last updated: 2021-07-15