Single-cell RNA-seq of lung cells following bacterial pneumonia with or without LIF blockade
Source: NCBI BioProject (ID PRJNA745341)
Source: NCBI BioProject (ID PRJNA745341)
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Project name: Single-cell RNA-seq of lung cells following bacterial pneumonia with or without LIF blockade
Description: C57BL/6 mice were intratracheally challenged for 24 hours with saline or E. coli (1 x 10^6 CFU) with or without LIF neutralization. Left lobes were collected and digested into single-cell suspensions for FACS. Equal ratios of CD45+ (leukocytes), CD45-EpCAM+ (epithelium), and CD45-EpCAM- (other) cells were pooled from a mouse undergoing each condition.Overall design: Sorted whole lung C57BL/6 mouse cells 24 hours after IgG saline, IgG E. coli, or anti-LIF E. coli
Data type: Transcriptome or Gene expression
Sample scope: Multiisolate
Relevance: ModelOrganism
Organization: BU Single Cell Sequencing Core
Last updated: 2021-07-10