Project name: Single cell transcriptome analysis of human oocyte aging

Description: To investigate the effects of women age on oocyte developmental competency, we used single-cell RNA sequencing to detect global gene transcriptome and identify key genes affected by advanced age in human mature oocytes.Overall design: We isolated mRNA from MII oocytes obtained from ICSI patients (six oocytes from younger (<30 years) and six oocytes from older (≥40 years) patients for scRNA-seq.

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: Medical

Last updated: 2020-07-27