Description: We performed a comparative evaluation of second (Illumina) and third (Oxford Nanopore Technologies) generation sequencing technologies for 16S targeted genomics using a well-characterized reference sample (ZymoBIOMICS Microbial Community DNA Standard). Different variable regions were amplified using the Illumina MiSeq and the whole 16S rRNA gene was amplified using the MinION. Classification accuracy was determined for different workflows using different software.

Data type: raw sequence reads

Sample scope: Multispecies

Relevance: Medical

Last updated: 2019-11-04