ZFX acts as a transcriptional activator in multiple types of human tumors by binding downstream of transcription start sites at the majority of CpG island promoters
Source: NCBI BioProject (ID PRJNA398222)
Source: NCBI BioProject (ID PRJNA398222)
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Project name: Homo sapiens
Description: We performed ChIP-seq in four cancer cell lines to identify ZFX binding sites throughout the human genome. We also performed RNA-seq analysis after knockdown of ZFX by siRNA in prostate and breast cancer cells. Using Nucleosome Occupancy and Methylome Sequencing (NOMe-seq), we show that ZFX binds between the open chromatin region at the TSS and the first downstream nucelosome, suggesting that ZFX may play a critical role in promoter architecture. We also showed that ZNF711 may function redundantly with ZFX in MCF7 by performing ZNF711 ChIP-seq and RNA-seq analysis after knockdown of ZFX, ZNF711, and both ZFX and ZNF711.Overall design: ChIP-seq, NOMe-seq and RNA-seq
Data type: Other
Sample scope: Multiisolate
Relevance: Medical
Organization: University of Southern California
Last updated: 2017-08-14