Project name: Saccharomyces cerevisiae

Description: This experiment compares mRNA expression in cells with and without mitochondrial DNA (ρ+ and ρ0 cells respectively). We used global RNA profiling to measure mRNA expression in ρ+ and ρ0 cells harvested at log phase from synthetic medium with glucose (CM + 2% glucose, "CMD"), and at 4 hours after transfer to the same medium without glucose (CM).This profiling provided new clues about the ways in which nutirent signaling is reprogrammed in cells lacking mitocondrial DNA.Overall design: mRNA expression was assessed by analyzing the RNA from three biological replicates obtained under four conditions: ρ+ cells in glucose, ρ+ cells no glucose, ρ0 cells in glucose, ρ0 cells no glucose.

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: ModelOrganism

Last updated: 2013-07-18