Project name: Bacillus anthracis

Description: The effect of factors that affect toxin production i.e. glucose, bicarbonate and growth temperature was investigated by transcriptional profilling using microarrays.Overall design: Sequential environmental perturbations were carried out in R media, wherein glucose was replaced by glycerol (0.25%) [referred as 'R(-)Glucose'] or no bicarbonate [referred as 'R(-)Bicarbonate'] was added or the growth temperature was 28°C instead of 37°C [referred as 'R-28C']. The strain was grown in a chemically defined media called R medium in the presence of 5%CO2 at 37°C to simulate host like conditions and induce toxin production (referred as ‘R-Full’), which is used as control in this study.

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: Medical

Last updated: 2013-02-20