Effect of hIAPP aggregation on gene expression by mouse bone marrow-derived macrophages after 12 hours
Source: NCBI BioProject (ID PRJNA131753)
Source: NCBI BioProject (ID PRJNA131753)
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Project name: Mus musculus
Description: The purpose of this experiment was to determine changes in gene expression by bone marrow-derived macrophages (BMDMs) treated with synthetic human vs. rodent islet amyloid polypeptide (IAPP). Synthetic human IAPP at 15 uM aggregates to form fibrils in vitro, whereas rodent IAPP is non-amyloidogenic. We hypothesized that interaction of macrophages with human IAPP aggregates can activate pro-inflammatory signalling pathways in macrophages, as described for other amyloidogenic peptides.Overall design: This array includes eight samples from one experiment, with two groups of four replicates each. Each replicate represents BMDMs from one well of a 24-well plate. The control group was treated with 15 uM rat IAPP for 12 hours prior to total RNA extraction; the experimental group was treated with 15 uM human IAPP.
Data type: Transcriptome or Gene expression
Sample scope: Multiisolate
Relevance: ModelOrganism
Organization: Dr. C. Bruce Verchere, Pathology and Laboratory Medicine, Child and Family Research Institute, University of British Columbia
Literatures
- PMID: 21813778
Release date: 2010-08-11
Last updated: 2010-08-10