Project name: Meiotic recombination initiation hotspots in Arabidopsis genes and transposons (MNase-seq)

Description: To analyse nucleosome positioning and occupancy we performed micrococcal nuclease digestion of Arabidopsis wild type (Col-0) chromatin and gel purified the resulting ~150 bp mononucleosomal DNA band. This DNA was used to generate a library and paired-end sequencing performed (MNase-seq). To further examine influence of SWR1 chromatin remodelling complex and DNA methylation on nucleosome occupancy, we repeated MNase-seq in Aarabidopsis arp6 and met1 mutant.

Data type: Other

Sample scope: Monoisolate

Last updated: 2017-06-06