Amplicon sequencing of CRISPR/Cas9 edited Lbx-A gene in lampreys.
Source: NCBI BioProject (ID PRJDB9194)
Source: NCBI BioProject (ID PRJDB9194)
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Project name: Lethenteron camtschaticum
Description: To clarify which of the lamprey larval muscles require function of Lbx-A during development, we disrupted LjLbx-A locus during embryogenesis, using CRISPR/Cas9-mediated gene editing. In order to confirm the effect of gene editing, amplicon library was constructed using a multiplexed method, and DNA sequencing was performed by Illumina MiSeq.
Data type: Targeted Locus (Loci)
Sample scope: Multiisolate
Literatures
- PMID: 32907560
Release date: 2020-08-15
Last updated: 2020-01-09