The nucleotide sequence of bacteriophage T5 glutamine transfer RNA.
Biochim Biophys Acta, 1984/7/18;782(3):313-9.
Shlyapnikov MG, Kaliman AV, Kazantsev SI, Kryukov VM, Bayev AA
PMID: 6733112
Abstract
Uniformly 32P-labeled phage-specific tRNAGln has been isolated from bacteriophage T5-infected Escherichia coli cells and its nucleotide sequence has been determined using thin-layer chromatography on cellulose to fractionate the oligonucleotides. The sequence is: pUGGGGAUUAGCUUAGCUUGGCCUAAAGCUUCGGCCUUUGAAG psi CGAGAUCAUUGGT psi CAAAUCCAAUAUCCCCUGCCAOH. The main feature of this tRNA is the absence of Watson-Crick pairing between the 5'-terminal base and the fifth base from its 3'-end. The structure of tRNA was confirmed by DNA sequencing of its gene.
MeSH terms
Base Sequence; DNA, Viral; Genes; Genes, Viral; Glutamine; Nucleic Acid Conformation; RNA, Transfer; T-Phages
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