A reference single-cell regulomic and transcriptomic map of cynomolgus monkeys.
Nat Commun, 2022/07/13;13(1):4069.
Qu J[1], Yang F[1], Zhu T[1], Wang Y[2], Fang W[1], Ding Y[1], Zhao X[1], Qi X[3], Xie Q[2], Chen M[4], Xu Q[1], Xie Y[5], Sun Y[6, 7], Chen D[8]
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PMID: 35831300DOI: 10.1038/s41467-022-31770-x
Impact factor: 17.694
Abstract
Non-human primates are attractive laboratory animal models that accurately reflect both developmental and pathological features of humans. Here we present a compendium of cell types across multiple organs in cynomolgus monkeys (Macaca fascicularis) using both single-cell chromatin accessibility and RNA sequencing data. The integrated cell map enables in-depth dissection and comparison of molecular dynamics, cell-type compositions and cellular heterogeneity across multiple tissues and organs. Using single-cell transcriptomic data, we infer pseudotime cell trajectories and cell-cell communications to uncover key molecular signatures underlying their cellular processes. Furthermore, we identify various cell-specific cis-regulatory elements and construct organ-specific gene regulatory networks at the single-cell level. Finally, we perform comparative analyses of single-cell landscapes among mouse, monkey and human. We show that cynomolgus monkey has strikingly higher degree of similarities in terms of immune-associated gene expression patterns and cellular communications to human than mouse. Taken together, our study provides a valuable resource for non-human primate cell biology.
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