Chromosome-Level Genome Assembly of the Green Peafowl (Pavo muticus).
Genome Biol Evol, 2022/2/02;
Zhang X[1], Lin C[2], Li H[3, 4], Liu S[1], Wang Q[3, 4], Yang S[5], Shi M[3, 4], Sahu SK[3], Zhu Y[3, 4], Wang J[3], Huang J[3], Hu Y[6, 7], Yu J[6, 7], Zhang S[6, 7], Li G[8], Guan W[9], Lu H[6, 7], Lan T[3], Xu Y[1]
Affiliations
PMID: 35106558DOI: 10.1093/gbe/evac015
Impact factor: 4.065
Abstract
The green peafowl (Pavo muticus) is facing a high risk of extinction due to the long-term and widespread threats of poaching and habitat conversion. Here, we present a high-quality chromosome-level genome assembly of the green peafowl with high contiguity and accuracy assembled by PacBio sequencing, DNBSEQ short-read sequencing, and Hi-C sequencing technologies. The final genome size was estimated to be 1.049 Gb, while 1.042 Gb of the genome was assigned to 27 pseudo-chromosomes. The scaffold N50 length was 75.5 Mb with a complete BUSCO score of 97.6%. We identified W and Z chromosomes and validated them by resequencing 14 additional individuals. Totally, 167.04 Mb repetitive elements were identified in the genome, accounting for 15.92% of the total genome size. We predicted 14,935 protein-coding genes, among which 14,931 genes were functionally annotated. This is the most comprehensive and complete de novo assembly of the Pavo Genus, and it will serve as a valuable resource for future green peafowl ecology, evolution, and conservation studies.
Keywords: PacBio sequencing; chromosome-level genome assembly; conservation; green peafowl
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