Comparing Arabidopsis receptor kinase and receptor protein-mediated immune signaling reveals BIK1-dependent differences.
New Phytol, 2019/03;221(4):2080-2095.
Wan WL[1], Zhang L[1], Pruitt R[1], Zaidem M[2, 3], Brugman R[4], Ma X[5], Krol E[6, 7], Perraki A[8, 9], Kilian J[10], Grossmann G[4], Stahl M[10], Shan L[5], Zipfel C[8], van Kan JAL[11], Hedrich R[6], Weigel D[2], Gust AA[1], Nürnberger T[1, 12]
Affiliations
PMID: 30252144DOI: 10.1111/nph.15497
Impact factor: 10.323
Abstract
Pattern recognition receptors (PRRs) sense microbial patterns and activate innate immunity against attempted microbial invasions. The leucine-rich repeat receptor kinases (LRR-RK) FLS2 and EFR, and the LRR receptor protein (LRR-RP) receptors RLP23 and RLP42, respectively, represent prototypical members of these two prominent and closely related PRR families. We conducted a survey of Arabidopsis thaliana immune signaling mediated by these receptors to address the question of commonalities and differences between LRR-RK and LRR-RP signaling. Quantitative differences in timing and amplitude were observed for several early immune responses, with RP-mediated responses typically being slower and more prolonged than those mediated by RKs. Activation of RLP23, but not FLS2, induced the production of camalexin. Transcriptomic analysis revealed that RLP23-regulated genes represent only a fraction of those genes differentially expressed upon FLS2 activation. Several positive and negative regulators of FLS2-signaling play similar roles in RLP23 signaling. Intriguingly, the cytoplasmic receptor kinase BIK1, a positive regulator of RK signaling, acts as a negative regulator of RP-type immune receptors in a manner dependent on BIK1 kinase activity. Our study unveiled unexpected differences in two closely related receptor systems and reports a new negative role of BIK1 in plant immunity.
Keywords: Arabidopsis; immune receptor; immune signaling comparison; plant immunity; receptor kinase; receptor protein
MeSH terms
Arabidopsis Proteins; Flagellin; Genotype; Peptides; Phosphorylation; Plant Growth Regulators; Plant Immunity; Protein Kinases; Protein Serine-Threonine Kinases; Reactive Oxygen Species; Receptors, Pattern Recognition; Salicylic Acid; Sesquiterpenes; Signal Transduction; Transcription, Genetic
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