Single-nucleotide resolution dynamic repair maps of UV damage in Saccharomyces cerevisiae genome.
Proc Natl Acad Sci U S A, 2018/04/10;115(15):E3408-E3415.
Li W[1], Adebali O[1], Yang Y[1], Selby CP[1], Sancar A[2]
Affiliations
PMID: 29581276DOI: 10.1073/pnas.1801687115
Impact factor: 12.779
Abstract
We have adapted the eXcision Repair-sequencing (XR-seq) method to generate single-nucleotide resolution dynamic repair maps of UV-induced cyclobutane pyrimidine dimers and (6-4) pyrimidine-pyrimidone photoproducts in the Saccharomyces cerevisiae genome. We find that these photoproducts are removed from the genome primarily by incisions 13-18 nucleotides 5' and 6-7 nucleotides 3' to the UV damage that generate 21- to 27-nt-long excision products. Analyses of the excision repair kinetics both in single genes and at the genome-wide level reveal strong transcription-coupled repair of the transcribed strand at early time points followed by predominantly nontranscribed strand repair at later stages. We have also characterized the excision repair level as a function of the transcription level. The availability of high-resolution and dynamic repair maps should aid in future repair and mutagenesis studies in this model organism.
Keywords: DNA damage; UV; XR-seq; excision repair; yeast
MeSH terms
DNA Damage; DNA Repair; DNA, Fungal; Genome, Fungal; Pyrimidine Dimers; Saccharomyces cerevisiae; Transcription, Genetic; Ultraviolet Rays
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