Multiple metabolic pathways for metabolism of l-tryptophan in Fusarium graminearum.
Can J Microbiol, 2017/11;63(11):921-927.
Luo K[1, 2], DesRoches CL[2], Johnston A[2], Harris LJ[2], Zhao HY[1], Ouellet T[2]
Affiliations
PMID: 28926717DOI: 10.1139/cjm-2017-0383
Impact factor: 3.226
Abstract
Fusarium graminearum is a plant pathogen that can cause the devastating cereal grain disease fusarium head blight in temperate regions of the world. Previous studies have shown that F. graminearum can synthetize indole-3-acetic acid (auxin) using l-tryptophan (L-TRP)-dependent pathways. In the present study, we have taken a broader approach to examine the metabolism of L-TRP in F. graminearum liquid culture. Our results showed that F. graminearum was able to transiently produce the indole tryptophol when supplied with L-TRP. Comparative gene expression profiling between L-TRP-treated and control cultures showed that L-TRP treatment induced the upregulation of a series of genes with predicted function in the metabolism of L-TRP via anthranilic acid and catechol towards the tricarboxylic acid cycle. It is proposed that this metabolic activity provides extra energy for 15-acetyldeoxynivalenol production, as observed in our experiments. This is the first report of the use of L-TRP to increase energy resources in a Fusarium species.
Keywords: Fusarium graminearum; global gene expression profiling; l-tryptophan metabolism; métabolisme du l-tryptophane; profil global d’expression génique; tryptophol
MeSH terms
Edible Grain; Fusarium; Gene Expression Profiling; Indoleacetic Acids; Indoles; Metabolic Networks and Pathways; Microarray Analysis; Trichothecenes; Triticum; Tryptophan
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