Single-cell RNA-seq reveals new types of human blood dendritic cells, monocytes, and progenitors.
Science, 2017/04/21;356(6335)
Villani AC[1, 2], Satija R[3, 4, 5], Reynolds G[6], Sarkizova S[3], Shekhar K[3], Fletcher J[6], Griesbeck M[7], Butler A[4, 5], Zheng S[4, 5], Lazo S[8], Jardine L[6], Dixon D[6], Stephenson E[6], Nilsson E[9], Grundberg I[9], McDonald D[6], Filby A[6], Li W[3, 2], De Jager PL[3, 10], Rozenblatt-Rosen O[3], Lane AA[3, 8], Haniffa M[11, 12], Regev A[1, 13, 14], Hacohen N[1, 2]
Affiliations
PMID: 28428369DOI: 10.1126/science.aah4573
Impact factor: 63.714
Abstract
Dendritic cells (DCs) and monocytes play a central role in pathogen sensing, phagocytosis, and antigen presentation and consist of multiple specialized subtypes. However, their identities and interrelationships are not fully understood. Using unbiased single-cell RNA sequencing (RNA-seq) of ~2400 cells, we identified six human DCs and four monocyte subtypes in human blood. Our study reveals a new DC subset that shares properties with plasmacytoid DCs (pDCs) but potently activates T cells, thus redefining pDCs; a new subdivision within the CD1C+ subset of DCs; the relationship between blastic plasmacytoid DC neoplasia cells and healthy DCs; and circulating progenitor of conventional DCs (cDCs). Our revised taxonomy will enable more accurate functional and developmental analyses as well as immune monitoring in health and disease.
MeSH terms
Adult; Antigen Presentation; Classification; Dendritic Cells; Female; Gene Expression Profiling; Humans; Lymphocyte Activation; Male; Monitoring, Immunologic; Monocytes; Neoplasms; Sequence Analysis, RNA; Single-Cell Analysis; T-Lymphocytes; Transcriptome; Young Adult
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