Department of Microbiology, University of Washington, Seattle, Washington, USA.
Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
European Molecular Biology Laboratory, European Bioinformatics Institute, Hinxton, Cambridge, UK.
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, Wisconsin, USA.
1] Department of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA. . [2] Molecular and Cellular Biology Program, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA.
1] Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA. [2] MRC Functional Genomics Unit, University of Oxford, xford, UK (D.B.); Biogen Idec, Cambridge, Massachusetts, USA (M.L.); Division of Biology, California Institute of Technology, Pasadena, California, USA (P.R.); Vertebrate and Health Genomics, The Genome Analysis Center, Norwich, UK (F.D.P.).
1] Department of Computer Science, Swiss Federal Institute of Technology (ETH Zurich), Zurich, Switzerland. [2] Swiss Institute of Bioinformatics, Zurich, Switzerland.
Department of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA. .
Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Department of Biological Statistics and Computational Biology, Cornell University, Ithaca, New York, USA.
Department of Wildlife Ecology and Conservation, University of Florida, Gainesville, Florida, USA.
1] European Molecular Biology Laboratory, European Bioinformatics Institute, Hinxton, Cambridge, UK. [2] Department of Genetics, Evolution and Environment, University College London, London, UK. [3] Department of Computer Science, University College London, London, UK.
1] Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, Wisconsin, USA. [2] ERATO Infection-Induced Host Responses Project, Japan Science and Technology Agency, Saitama, Japan. [3] Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan. [4] Department of Special Pathogens, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo, Japan. [5] Laboratory of Bioresponses Regulation, Department of Biological Responses, Institute for Virus Research, Kyoto University, Kyoto, Japan.
1] Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA. [2] Science for Life Laboratory, Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden.
1] Department of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA. . [2] Center for Gene Therapy, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA.
1] Department of Microbiology, University of Washington, Seattle, Washington, USA. [2] Washington National Primate Research Center, Seattle, Washington, USA.
The domestic ferret (Mustela putorius furo) is an important animal model for multiple humanrespiratory diseases. It is considered the 'gold standard' for modeling humaninfluenza virus infection and transmission. Here we describe the 2.41 Gb draft genome assembly of the domestic ferret, constituting 2.28 Gb of sequence plus gaps. We annotated 19,910 protein-coding genes on this assembly using RNA-seq data from 21 ferret tissues. We characterized the ferret host response to two influenza virusinfections by RNA-seq analysis of 42 ferret samples from influenza time-course data and showed distinct signatures in ferrettrachea and lung tissues specific to 1918 or 2009 human pandemic influenza virusinfections. Using microarray data from 16 ferret samples reflecting cystic fibrosisdisease progression, we showed that transcriptional changes in the CFTR-knockout ferret lung reflect pathways of early disease that cannot be readily studied in human infants with cystic fibrosis disease.
