Inactivation of the GacA response regulator in Pseudomonas fluorescens Pf-5 has far-reaching transcriptomic consequences.
Environ Microbiol, 2010/4;12(4):899-915.
Hassan KA[1], Johnson A, Shaffer BT, Ren Q, Kidarsa TA, Elbourne LD, Hartney S, Duboy R, Goebel NC, Zabriskie TM, Paulsen IT, Loper JE
Affiliations
PMID: 20089046DOI: 10.1111/j.1462-2920.2009.02134.x
Impact factor: 5.476
Abstract
The GacS/GacA signal transduction system is a central regulator in Pseudomonas spp., including the biological control strain P. fluorescens Pf-5, in which GacS/GacA controls the production of secondary metabolites and exoenzymes that suppress plant pathogens. A whole genome oligonucleotide microarray was developed for Pf-5 and used to assess the global transcriptomic consequences of a gacA mutation in P. fluorescens Pf-5. In cultures at the transition from exponential to stationary growth phase, GacA significantly influenced transcript levels of 635 genes, representing more than 10% of the 6147 annotated genes in the Pf-5 genome. Transcripts of genes involved in the production of hydrogen cyanide, the antibiotic pyoluteorin and the extracellular protease AprA were at a low level in the gacA mutant, whereas those functioning in siderophore production and other aspects of iron homeostasis were significantly higher in the gacA mutant than in wild-type Pf-5. Notable effects of gacA inactivation were also observed in the transcription of genes encoding components of a type VI secretion system and cytochrome c oxidase subunits. Two novel gene clusters expressed under the control of gacA were identified from transcriptome analysis, and we propose global-regulator-based genome mining as an approach to decipher the secondary metabolome of Pseudomonas spp.
MeSH terms
Bacterial Proteins; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Genome, Bacterial; Multigene Family; Mutation; Oligonucleotide Array Sequence Analysis; Pseudomonas fluorescens; RNA, Bacterial; Sequence Deletion
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