Expression profile analysis of aorta-gonad-mesonephros region-derived stromal cells reveals genes that regulate hematopoiesis.
Biochem Biophys Res Commun, 2008/12/05;377(1):205-9.
Nagao K[1], Ohta T, Hinohara A, Tahara T, Hagiwara T, Maeda Y, Yoneya T, Sohma Y, Heike T, Nakahata T, Inagaki Y, Nishikawa M
Affiliations
PMID: 18840400DOI: 10.1016/j.bbrc.2008.09.123
Impact factor: 3.322
Abstract
The aorta-gonad-mesonephros (AGM) region is involved in the generation and maintenance of the first definitive hematopoietic stem cells (HSCs). A mouse AGM-derived cell line, AGM-S3, was shown to support the development of HSCs. To elucidate the molecular mechanisms regulating early hematopoiesis, we obtained subclones from AGM-S3, one of which was hematopoiesis supportive (S3-A9) and the other one of which was non-supportive (S3-A7), and we analyzed their gene expression profiles by gene chip analysis. In the present study, we found that Glypican-1 (GPC1) was highly expressed in the supportive subclone AGM-S3-A9. Over-expression of GPC1 in non-supportive cells led to the proliferation of progenitor cells in human cord blood when cocultured with the transfected-stromal cells. Thus, GPC1 may have an important role in the establishment of a microenvironment that supports early events in hematopoiesis.
MeSH terms
Animals; Aorta; Cell Line; Cell Proliferation; Fetal Blood; Gene Expression Profiling; Gene Expression Regulation, Developmental; Glypicans; Gonads; Hematopoiesis; Hematopoietic Stem Cells; Humans; Mesonephros; Mice; Stromal Cells; Transfection
More resources
EndNote: Download