Pituitary adenylate cyclase activating peptide induces cGMP-mediated relaxation in guinea-pig airways.
Pulm Pharmacol Ther, 1998;11(4):281-5.
Saotome M[1], Uchida Y, Nomura A, Endo T, Hasegawa S
Affiliations
PMID: 10101745
Impact factor: 3.282
Abstract
The mechanism of relaxation of the guinea-pig trachea induced by pituitary adenylate cyclase activating peptide (PACAP)-27 was investigated. We examined whether modulators of nitric oxide (NO) and carbon monoxide (CO) affect PACAP-induced response of tracheal strips in vitro. Pretreatment with N omega-nitro-L-arginine methyl ester hydrochloride (L-NAME) and L-arginine (L-arg) had no effect, while 1H-[1,2,4] oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), haemoglobin and zinc protoporphyrin IX (ZnPP-9) partially abolished the PACAP-induced relaxation. PACAP-27 elevated cyclic GMP level in airway smooth muscle tissue. These results indicate that PACAP-27 not only induces cyclic AMP-mediated responses, but also cyclic GMP-mediated responses in the airway. In addition, CO is related to the PACAP-induced elevation of cGMP level in the tracheal tissue.
MeSH terms
Analysis of Variance; Animals; Carbon Monoxide; Cyclic GMP; Guinea Pigs; In Vitro Techniques; Muscle Relaxation; Muscle, Smooth; Neuropeptides; Nitric Oxide; Pituitary Adenylate Cyclase-Activating Polypeptide; Trachea; Vasodilator Agents
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