We are constructing the comprehensive sample and genetic bank for children with medulloblastoma and other cerebellum disorders, which contains the bulk sequence for genome, epigenome and transcripts, as well as the single-nucleus multi-omics for transcriptomics, chromosome accessibility and spatial RNA expression. Furthermore, the Clinical information including the operation, radiation, et al. and the long term follow-up profiles.
Contact :doctorjiangtt@163.comlijiankang@genomics.cnhailong_liu@bjtth.org
Medulloblastoma (MB) constitutes the most common malignant brain tumor in childhood, for which comprehensive management is operation followed by chemotherapy and radiation. It has been well established that medulloblastoma (MB) comprises four molecular subgroups including WNT, SHH and Group 3/4 (or non-SHH/non-WNT) based on the latest version of WHO classification. Recurrences (local or disseminated lesions) are observed among nearly all MB, along the neuraxis including leptomeninges, ventricular system and cerebrospinal parenchyma signifying a formidable treatment challenge. The mixed pattern of divergent cell populations hampered the explicit knowledge of MB subgroup evolution. Moreover, the spatially resolved investigations of specific cell populations and gene modules remains rudimentary.
We comprehensively analyze single-nucleus RNA expression, chromatin accessibility and spatial transcriptomic profiling from 51 human medulloblastomas spanning four molecular subgroups, complemented with the bulk whole genome and RNA sequencing data across 322 human samples. We comprehensively interrogated the composition of medulloblastoma microenvironment and spatially described the developmental trajectory from progenitor-like to differentiated malignant cells.
Chen A, Liao S, Cheng M, et al. Spatiotemporal transcriptomic atlas of mouse organogenesis using DNA nanoball-patterned arrays. Cell. 2022;185(10):1777-1792.e21. doi:10.1016/j.cell.2022.04.003
