Supplementary Figures

Supplementary Figure 1.

Heatmaps showing the correlation of zonation binning to the number (left) or choice (right) of zonation landmark genes.

Supplementary Figure 2.

Side-by-side comparison of newly identified zonated genes measured by Stereo-seq with RNAscope (Thrb, Fgfr1, Bmp4, Ackr4, Egfl7 and Wnt4) or immunofluorescence staining (SLC10A1 and LAMP2). The colors indicate the technique (red, Stereo-seq; blue, smFISH or immunofluorescence staining).