Hyperpolarised 13C-MRI identifies the emergence of a glycolytic cell population within intermediate-risk human prostate cancer.
IF: 17.694
Cited by: 17


Hyperpolarised magnetic resonance imaging (HP 13C-MRI) is an emerging clinical technique to detect [1-13C]lactate production in prostate cancer (PCa) following intravenous injection of hyperpolarised [1-13C]pyruvate. Here we differentiate clinically significant PCa from indolent disease in a low/intermediate-risk population by correlating [1-13C]lactate labelling on MRI with the percentage of Gleason pattern 4 (%GP4) disease. Using immunohistochemistry and spatial transcriptomics, we show that HP 13C-MRI predominantly measures metabolism in the epithelial compartment of the tumour, rather than the stroma. MRI-derived tumour [1-13C]lactate labelling correlated with epithelial mRNA expression of the enzyme lactate dehydrogenase (LDHA and LDHB combined), and the ratio of lactate transporter expression between the epithelial and stromal compartments (epithelium-to-stroma MCT4). We observe similar changes in MCT4, LDHA, and LDHB between tumours with primary Gleason patterns 3 and 4 in an independent TCGA cohort. Therefore, HP 13C-MRI can metabolically phenotype clinically significant disease based on underlying metabolic differences in the epithelial and stromal tumour compartments.


Spatial Transcriptomics

MeSH terms

Aged, 80 and over
Cohort Studies
Epithelial Cells
L-Lactate Dehydrogenase
Magnetic Resonance Imaging
Middle Aged
Monocarboxylic Acid Transporters
Muscle Proteins
Prospective Studies
Prostatic Neoplasms
Pyruvic Acid
Stromal Cells


Sushentsev, Nikita
McLean, Mary A
Warren, Anne Y
Benjamin, Arnold J V
Brodie, Cara
Frary, Amy
Gill, Andrew B
Jones, Julia
Kaggie, Joshua D
Lamb, Benjamin W
Locke, Matthew J
Miller, Jodi L
Mills, Ian G
Priest, Andrew N
Robb, Fraser J L
Shah, Nimish
Schulte, Rolf F
Graves, Martin J
Gnanapragasam, Vincent J
Brindle, Kevin M
Barrett, Tristan
Gallagher, Ferdia A

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