Mass Spectrometry Imaging of Bio-oligomer Polydispersity in Plant Tissues by Laser Desorption Ionization from Silicon Nanopost Arrays.
PMID:33529427
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IF: 16.823
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Cited by: 17
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Abstract

Mass spectrometry imaging (MSI) enables simultaneous spatial mapping for diverse molecules in biological tissues. Matrix-assisted laser desorption ionization (MALDI) mass spectrometry (MS) has been a mainstream MSI method for a wide range of biomolecules. However, MALDI-MSI of biological homopolymers used for energy storage and molecular feedstock is limited by, e.g., preferential ionization for certain molecular classes. Matrix-free nanophotonic ionization from silicon nanopost arrays (NAPAs) is an emerging laser desorption ionization (LDI) platform with ultra-trace sensitivity and molecular imaging capabilities. Here, we show complementary analysis and MSI of polyhydroxybutyric acid (PHB), polyglutamic acid (PGA), and polysaccharide oligomers in soybean root nodule sections by NAPA-LDI and MALDI. For PHB, number and weight average molar mass, polydispersity, and oligomer size distributions across the tissue section and in regions of interest were characterized by NAPA-LDI-MSI.

Keywords

NAPA-LDI-MS
MALDI-MSI
MALDI
biopolymers
imaging
mass spectrometry
nanopost arrays
oligomer size distribution

MeSH terms

Hydroxybutyrates
Molecular Imaging
Nanostructures
Plant Roots
Polyesters
Polyglutamic Acid
Polysaccharides
Silicon
Glycine max
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Authors

Samarah, Laith Z
Tran, Tina H
Stacey, Gary
Vertes, Akos

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