Identification of region-specific astrocyte subtypes at single cell resolution.
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IF: 17.694
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Cited by: 368
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Abstract

Astrocytes, a major cell type found throughout the central nervous system, have general roles in the modulation of synapse formation and synaptic transmission, blood-brain barrier formation, and regulation of blood flow, as well as metabolic support of other brain resident cells. Crucially, emerging evidence shows specific adaptations and astrocyte-encoded functions in regions, such as the spinal cord and cerebellum. To investigate the true extent of astrocyte molecular diversity across forebrain regions, we used single-cell RNA sequencing. Our analysis identifies five transcriptomically distinct astrocyte subtypes in adult mouse cortex and hippocampus. Validation of our data in situ reveals distinct spatial positioning of defined subtypes, reflecting the distribution of morphologically and physiologically distinct astrocyte populations. Our findings are evidence for specialized astrocyte subtypes between and within brain regions. The data are available through an online database (https://holt-sc.glialab.org/), providing a resource on which to base explorations of local astrocyte diversity and function in the brain.

Keywords

Seurat
Gene Expression
RNAscope

MeSH terms

Animals
Astrocytes
Calcium Signaling
Cell Shape
Gene Expression Regulation
Mice, Inbred C57BL
Neurogenesis
Organ Specificity
RNA, Messenger
Single-Cell Analysis

Authors

Batiuk, Mykhailo Y
Martirosyan, Araks
Wahis, Jérôme
de Vin, Filip
Marneffe, Catherine
Kusserow, Carola
Koeppen, Jordan
Viana, João Filipe
Oliveira, João Filipe
Voet, Thierry
Ponting, Chris P
Belgard, T Grant
Holt, Matthew G

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