Angiogenic Gene Profiles in Laser-Microdissected Microvessels and Neurons from Ischemic Penumbra of Rat Brain.
IF: 2.866


Angiogenesis is induced immediately after cerebral ischemia and plays a pivotal role in the strategy against ischemic injury. We hypothesized that the coordinated interaction between microvessels and neurons was altered immediately after stroke, and microvessels and neurons would show the temporal specificity of angiogenic gene profiles after cerebral ischemia. Microvessels and neurons were harvested in the ischemic penumbra of rat brain using the PixCell II laser capture microdissection (LCM) instrument. After RNA isolation, T7 and gene-specific primer RNA linear amplification were performed, and angiogenic functional grouping cDNA profiling was analyzed in LCM samples. cDNA microarray results showed there were 35 (36.46%) and 27 (28.13%) genes expression changes in the microvessels, while 25 (26.04%) and 31 (32.29%) genes were changed in the neurons at 2 h and 24 h after cerebral ischemia. Members of growth factors and receptors, cytokines and chemokines, adhesion molecules, matrix proteins, proteases, and inhibitors showed temporal and spatial differentiation in the microvessels and neurons after cerebral ischemia. This finding will help to understand the coordination and interaction between microvessels and neurons, and to elucidate the molecular mechanisms of angiogenesis after brain ischemic injury.


Gene Expression
Cerebral ischemia
Laser capture microdissection

MeSH terms

Gene Expression Profiling
Infarction, Middle Cerebral Artery
Laser Capture Microdissection
Neovascularization, Physiologic
Rats, Sprague-Dawley


Tian, Yingfang
Di, Yuanyuan
Zhang, Jianshui
Chen, Xinlin
Feng, Ting
Adu-Nti, Frank
Shi, Meimei
Fan, Juan
Zhang, Junfeng
Zhang, Pengbo
Liu, Yong

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