Highly Multiplexed Single-Cell Protein Profiling with Large-Scale Convertible DNA-Antibody Barcoded Arrays.
Highly multiplexed detection of proteins secreted by single cells is always challenging. Herein, a multiplexed in situ tagging technique based on single-stranded DNA encoded microbead arrays and multicolor successive imaging for assaying single-cell secreted proteins with high throughput and high sensitivity is presented. This technology is demonstrated to be capable of increasing the multiplexity exponentially. Upon integration with polydimethylsiloxane microwells, this platform is applied to detect ten immune effector proteins from differentiated single macrophages stimulated with lipopolysaccharide. Significant heterogeneity is observed when the derived human primary macrophages are analyzed. This versatile technology is expected to open new opportunities in systems biology, immune regulation studies, signaling analysis, and molecular diagnostics.
1. Highly multiplexed tissue imaging using repeated oligonucleotide exchange reaction.
2. Highly Sensitive and Multiplexed In-Situ Protein Profiling with Cleavable Fluorescent Streptavidin.
3. New tools for pathology: a user's review of a highly multiplexed method for in situ analysis of protein and RNA expression in tissue.
4. High-throughput single-cell gene-expression profiling with multiplexed error-robust fluorescence in situ hybridization.
5. Highly Multiplexed Single-Cell Protein Profiling with Large-Scale Convertible DNA-Antibody Barcoded Arrays.
1. Highly multiplexed and quantitative cell surface protein profiling using genetically barcoded antibodies
2. Single cell RNA-seq of primary human glioblastomas
3. Spatially resolved, highly multiplexed RNA profiling in single cells
4. Single cell transcriptome analysis of human pancreas reveals transcriptional signatures of aging and somatic mutation patterns.