Step 1 Uploading data and Choosing the reference genome
    

e.g. , data1(1 sample, 323MB, hg38)

Note: MUST fastq format and NOT include '.' in filename.


Note Defining the case-control status
Group 1
Group 2

Step 2  

            Note: Default pipeline can be modified to meet job-specific requirement.

(1) Assessing sequence data quality

    •     

(2) Read filtering, trimming and alignment

  • filtering and trimming
          
  • alignment
          

(3) Post-mapping quality inspection of Ribo-seq data

    •                         

(4) Read count quantitation and Normalization

  • raw counts
          
  • normalized counts
               

(5) Detecting actively translated ORFs

  • gene level
          
  • exon level
               

(6) Differential translational efficiency analysis

    •     
Step 3  

            Note: Default parameters can be overwritten if required.


filtering and trimming
alignment
Footprint length
Differential cutoff
Threads