Taking the amino acid sequence search as an example, if we want to blast a T cell receptor CDR3 sequence(“CASSDGTGEAEAFF”) specific to Influenza antigen HA255 which were collected in TBAdb, we should choose “BLASTP” (If a nucleotide is interested, please choose “BLASTN”) tool:

The 1^st step: Input the sequence of your interest, which is “CASSDGTGEAEAFF” (You can also submit your query sequences with a fasta file). Please note to choose “Short BLASTP” task because the most of the sequences in PIRD are short.

The 2^nd step: Select PIRD as the target database.

The 3^rd step: Select the BLAST parameters as needed, or leave them as default.

The 4^th step: Submit BLASTP and wait for the result.

If you have logged in the database, you will also receive an e-mail notification:

The 5^th step: Check and download your results. In the detailed information, you can either download all sequences or select and download interested ones.