PMID- 36142482 OWN - NLM STAT- MEDLINE VI - 23 IP - 18 TI - Systematic Characterization of the OSCA Family Members in Soybean and Validation of Their Functions in Osmotic Stress. LA - eng PT - Journal Article PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 IS - 1422-0067 (Electronic) LID - 10570 [pii] LID - 10.3390/ijms231810570 [doi] FAU - Liu, Congge AU - Liu C AD - Longping Branch, College of Biology, Hunan University, Changsha 410125, China. AD - College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China. FAU - Wang, Hong AU - Wang H AD - State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 311401, China. FAU - Zhang, Yu AU - Zhang Y AD - College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China. FAU - Cheng, Haijing AU - Cheng H AD - College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China. FAU - Hu, Zhangli AU - Hu Z AUID- ORCID: 0000-0002-2596-8515 AD - College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China. FAU - Pei, Zhen-Ming AU - Pei ZM AD - Department of Biology, Duke University, Durham, NC 27708, USA. FAU - Li, Qing AU - Li Q AUID- ORCID: 0000-0001-7010-7776 AD - College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China. AD - State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 311401, China. IS - 1422-0067 (Linking) RN - SY7Q814VUP (Calcium) RN - 0 (Plant Proteins) SB - IM MH - *Arabidopsis/genetics/metabolism MH - Calcium/metabolism MH - Droughts MH - *Fabaceae/metabolism MH - Gene Expression Regulation, Plant MH - Osmotic Pressure MH - Phylogeny MH - Plant Proteins/metabolism MH - Glycine max/genetics/metabolism MH - Stress, Physiological/genetics OTO - NOTNLM OT - OSCA OT - calcium OT - osmosensor OT - osmotic stress OT - soybean PMC - PMC9500692 DCOM- 20221230 LR - 20231213 DP - 2022 Sep 12 DEP - 20220912 AB - Since we discovered OSCA1, a hyperosmolarity-gated calcium-permeable channel that acted as an osmosensor in Arabidopsis, the OSCA family has been identified genome-wide in several crops, but only a few OSCA members' functions have been experimentally demonstrated. Osmotic stress seriously restricts the yield and quality of soybean. Therefore, it is essential to decipher the molecular mechanism of how soybean responds to osmotic stress. Here, we first systematically studied and experimentally demonstrated the role of OSCA family members in the osmotic sensing of soybean. Phylogenetic relationships, gene structures, protein domains and structures analysis revealed that 20 GmOSCA members were divided into four clades, of which members in the same cluster may have more similar functions. In addition, GmOSCA members in clusters III and IV may be functionally redundant and diverged from those in clusters I and II. Based on the spatiotemporal expression patterns, GmOSCA1.6, GmOSCA2.1, GmOSCA2.6, and GmOSCA4.1 were extremely low expressed or possible pseudogenes. The remaining 16 GmOSCA genes were heterologously overexpressed in an Arabidopsis osca1 mutant, to explore their functions. Subcellular localization showed that most GmOSCA members could localize to the plasma membrane (PM). Among 16 GmOSCA genes, only overexpressing GmOSCA1.1, GmOSCA1.2, GmOSCA1.3, GmOSCA1.4, and GmOSCA1.5 in cluster I could fully complement the reduced hyperosmolality-induced [Ca2+]i increase (OICI) in osca1. The expression profiles of GmOSCA genes against osmotic stress demonstrated that most GmOSCA genes, especially GmOSCA1.1, GmOSCA1.2, GmOSCA1.3, GmOSCA1.4, GmOSCA1.5, GmOSCA3.1, and GmOSCA3.2, strongly responded to osmotic stress. Moreover, overexpression of GmOSCA1.1, GmOSCA1.2, GmOSCA1.3, GmOSCA1.4, GmOSCA1.5, GmOSCA3.1, and GmOSCA3.2 rescued the drought-hypersensitive phenotype of osca1. Our findings provide important clues for further studies of GmOSCA-mediated calcium signaling in the osmotic sensing of soybean and contribute to improving soybean drought tolerance through genetic engineering and molecular breeding.