PMID- 35683012 OWN - NLM STAT- MEDLINE VI - 23 IP - 11 TI - A Comprehensive Identification and Expression Analysis of VQ Motif-Containing Proteins in Sugarcane (Saccharum spontaneum L.) under Phytohormone Treatment and Cold Stress. LA - eng PT - Journal Article PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 IS - 1422-0067 (Electronic) LID - 6334 [pii] LID - 10.3390/ijms23116334 [doi] FAU - Liu, Ying AU - Liu Y AD - Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization, School of Life Science, Hunan University of Science and Technology, Xiangtan 411201, China. AD - Key Laboratory of Integrated Management of the Pests and Diseases on Horticultural Crops in Hunan Province/Key Laboratory of Ecological Remediation and Safe Utilization of Heavy Metal-Polluted Soils, College of Hunan Province, Xiangtan 411201, China. FAU - Liu, Xiaolan AU - Liu X AD - Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization, School of Life Science, Hunan University of Science and Technology, Xiangtan 411201, China. AD - Key Laboratory of Integrated Management of the Pests and Diseases on Horticultural Crops in Hunan Province/Key Laboratory of Ecological Remediation and Safe Utilization of Heavy Metal-Polluted Soils, College of Hunan Province, Xiangtan 411201, China. FAU - Yang, Dandan AU - Yang D AD - Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization, School of Life Science, Hunan University of Science and Technology, Xiangtan 411201, China. FAU - Yin, Ze AU - Yin Z AD - Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization, School of Life Science, Hunan University of Science and Technology, Xiangtan 411201, China. FAU - Jiang, Yaolan AU - Jiang Y AD - Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization, School of Life Science, Hunan University of Science and Technology, Xiangtan 411201, China. FAU - Ling, Hui AU - Ling H AD - College of Agriculture, Yulin Normal University, Yulin 537000, China. FAU - Huang, Ning AU - Huang N AD - College of Agriculture, Yulin Normal University, Yulin 537000, China. FAU - Zhang, Dawei AU - Zhang D AD - Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization, School of Life Science, Hunan University of Science and Technology, Xiangtan 411201, China. AD - Key Laboratory of Integrated Management of the Pests and Diseases on Horticultural Crops in Hunan Province/Key Laboratory of Ecological Remediation and Safe Utilization of Heavy Metal-Polluted Soils, College of Hunan Province, Xiangtan 411201, China. FAU - Wu, Jinfeng AU - Wu J AD - Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization, School of Life Science, Hunan University of Science and Technology, Xiangtan 411201, China. AD - Key Laboratory of Integrated Management of the Pests and Diseases on Horticultural Crops in Hunan Province/Key Laboratory of Ecological Remediation and Safe Utilization of Heavy Metal-Polluted Soils, College of Hunan Province, Xiangtan 411201, China. FAU - Liu, Lili AU - Liu L AD - Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization, School of Life Science, Hunan University of Science and Technology, Xiangtan 411201, China. AD - Key Laboratory of Integrated Management of the Pests and Diseases on Horticultural Crops in Hunan Province/Key Laboratory of Ecological Remediation and Safe Utilization of Heavy Metal-Polluted Soils, College of Hunan Province, Xiangtan 411201, China. FAU - Xu, Liping AU - Xu L AD - Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture/National Engineering Research Center for Sugarcane, Ministry of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China. FAU - Yan, Mingli AU - Yan M AD - Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization, School of Life Science, Hunan University of Science and Technology, Xiangtan 411201, China. AD - Crop Research Institute, Hunan Academy of Agricultural Sciences, Changsha 410000, China. FAU - Que, Youxiong AU - Que Y AUID- ORCID: 0000-0003-1111-5834 AD - Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture/National Engineering Research Center for Sugarcane, Ministry of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China. FAU - Zhou, Dinggang AU - Zhou D AUID- ORCID: 0000-0001-5665-9312 AD - Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization, School of Life Science, Hunan University of Science and Technology, Xiangtan 411201, China. AD - Key Laboratory of Integrated Management of the Pests and Diseases on Horticultural Crops in Hunan Province/Key Laboratory of Ecological Remediation and Safe Utilization of Heavy Metal-Polluted Soils, College of Hunan Province, Xiangtan 411201, China. AD - Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture/National Engineering Research Center for Sugarcane, Ministry of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China. IS - 1422-0067 (Linking) RN - 0 (Plant Growth Regulators) RN - 0 (Plant Proteins) SB - IM MH - Cold-Shock Response MH - Gene Expression Regulation, Plant MH - Phylogeny MH - Plant Growth Regulators/metabolism MH - Plant Proteins/metabolism MH - *Saccharum/genetics/metabolism MH - Stress, Physiological/genetics OTO - NOTNLM OT - Saccharum spontaneum OT - Transcriptome OT - VQ gene OT - cold stress OT - genome-wide analysis OT - phytohormone PMC - PMC9181594 DCOM- 20220613 LR - 20220716 DP - 2022 Jun 06 DEP - 20220606 AB - The VQ motif-containing proteins play a vital role in various processes such as growth, resistance to biotic and abiotic stresses and development. However, there is currently no report on the VQ genes in sugarcane (Saccharum spp.). Herein, 78 VQ genes in Saccharum spontaneum were identified and classified into nine subgroups (I-IX) by comparative genomic analyses. Each subgroup had a similar structural and conservative motif. These VQ genes expanded mainly through whole-genome segmental duplication. The cis-regulatory elements (CREs) of the VQ genes were widely involved in stress responses, phytohormone responses and physiological regulation. The RNA-seq data showed that SsVQ gene expression patterns in 10 different samples, including different developmental stages, revealed distinct temporal and spatial patterns. A total of 23 SsVQ genes were expressed in all tissues, whereas 13 SsVQ genes were not expressed in any tissues. Sequence Read Archive (SRA) data showed that the majority of SsVQs responded to cold and drought stress. In addition, quantitative real-time PCR analysis showed that the SsVQs were variously expressed under salicylic acid (SA), jasmonic acid (JA), abscisic acid (ABA) and cold treatment. This study conducted a full-scale analysis of the VQ gene family in sugarcane, which could be beneficial for the functional characterization of sugarcane VQ genes and provide candidate genes for molecular resistance breeding in cultivated sugarcane in the future.