PMID- 35627108 OWN - NLM STAT- MEDLINE VI - 13 IP - 5 TI - Epigenetic Regulation Mechanisms of the Cofilin-1 Gene in the Development and Differentiation of Bovine Primary Myoblasts. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Switzerland TA - Genes (basel) JT - Genes JID - 101551097 IS - 2073-4425 (Electronic) LID - 723 [pii] LID - 10.3390/genes13050723 [doi] FAU - Sun, Yujia AU - Sun Y AUID- ORCID: 0000-0002-2772-7758 AD - Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, China. AD - Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou 225009, China. FAU - Ma, Yaoyao AU - Ma Y AD - Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, China. AD - Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou 225009, China. FAU - Zhao, Tianqi AU - Zhao T AD - Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, China. AD - Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou 225009, China. FAU - Li, Mingxun AU - Li M AD - Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou 225009, China. FAU - Mao, Yongjiang AU - Mao Y AD - Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou 225009, China. FAU - Yang, Zhangping AU - Yang Z AUID- ORCID: 0000-0002-5479-8447 AD - Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, China. AD - Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou 225009, China. IS - 2073-4425 (Linking) RN - 0 (Actin Depolymerizing Factors) RN - 0 (MicroRNAs) SB - IM MH - Actin Depolymerizing Factors/genetics/metabolism MH - Animals MH - Cattle MH - *Epigenesis, Genetic/genetics MH - *MicroRNAs/metabolism MH - Muscle Development/genetics MH - Myoblasts/metabolism OTO - NOTNLM OT - bovine primary myoblasts OT - cell differentiation OT - cofilin-1 OT - epigenetic regulation PMC - PMC9140398 DCOM- 20220531 LR - 20220917 DP - 20220421 DEP - 20220421 AB - As the quality of beef products has received increasing attention, it is essential to explore the underlying transcriptional and epigenetic mechanisms of meat traits. Our project uses Qinchuan cattle as the research subject. First, we examined the spatiotemporal expression pattern of the CFL1 gene in a panel of fetal bovine, calf, and adult cattle samples. Then, we performed DNA methylation experiments of CFL1 on myogenesis and muscle maturation using the BSP amplification and COBRA sequencing techniques and found that high DNA methylation levels showed low expression levels. Next, we performed an assay between bta-miR-182 and the CFL1 gene and demonstrated that miR-182 could promote bovine primary myoblast differentiation by negatively regulated the expression of CFL1. Finally, we constructed an adenovirus overexpression and interference vector and found that CFL1 could suppress the differentiation of bovine primary myoblasts. In summary, our experiment comprehensively analyzes the epigenetic regulation mechanisms of the CFL1 gene in the development and differentiation of bovine primary myoblasts. This has far-reaching significance for improving the meat production and meat quality of Qinchuan cattle. This can provide reliable data support and a theoretical research basis for the rapid and efficient breeding selection of local yellow cattle and the genetic improvement of meat quality.