PMID- 35252817 OWN - NLM STAT- PubMed-not-MEDLINE VI - 25 IP - 3 TI - Spatially resolved transcriptomics of high-grade serous ovarian carcinoma. PG - 103923 CI - © 2022 The Author(s). LA - eng PT - Journal Article PL - United States TA - Iscience JT - iScience JID - 101724038 IS - 2589-0042 (Electronic) LID - 10.1016/j.isci.2022.103923 [doi] FAU - Stur, Elaine AU - Stur E AD - Department of Gynecologic Oncology and Reproductive Medicine, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX 77054, USA. FAU - Corvigno, Sara AU - Corvigno S AD - Department of Gynecologic Oncology and Reproductive Medicine, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX 77054, USA. FAU - Xu, Mingchu AU - Xu M AD - Department of Genomic Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX 77054, USA. FAU - Chen, Ken AU - Chen K AD - Department of Bioinformatics and Computational Biology, The University of Texas MD Anderson Cancer Center, Houston, TX 77054, USA. FAU - Tan, Yukun AU - Tan Y AD - Department of Bioinformatics and Computational Biology, The University of Texas MD Anderson Cancer Center, Houston, TX 77054, USA. FAU - Lee, Sanghoon AU - Lee S AD - Department of Systems Biology, The University of Texas MD Anderson Cancer Center, Houston, TX 77054, USA. FAU - Liu, Jinsong AU - Liu J AD - Department of Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX 77054, USA. FAU - Ricco, Emily AU - Ricco E AD - Genomic and RNA Profiling Core, Baylor College of Medicine, Houston, TX 77030, USA. FAU - Kraushaar, Daniel AU - Kraushaar D AD - Genomic and RNA Profiling Core, Baylor College of Medicine, Houston, TX 77030, USA. FAU - Castro, Patricia AU - Castro P AD - Pathology and Histology Core, Baylor College of Medicine, Houston, TX 77030, USA. FAU - Zhang, Jianhua AU - Zhang J AD - Department of Genomic Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX 77054, USA. FAU - Sood, Anil K AU - Sood AK AD - Department of Gynecologic Oncology and Reproductive Medicine, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX 77054, USA. AD - Center for RNA Interference and Non-Coding RNA, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX 77054, USA. IS - 2589-0042 (Linking) OTO - NOTNLM OT - Omics OT - Oncology OT - Pathology PMC - PMC8891954 LR - 20240303 DP - 2022 Mar 18 DEP - 20220214 AB - Bulk and single-cell RNA sequencing do not provide full characterization of tissue spatial diversity in cancer samples, and currently available in situ techniques (multiplex immunohistochemistry and imaging mass cytometry) allow for only limited analysis of a small number of targets. The current study represents the first comprehensive approach to spatial transcriptomics of high-grade serous ovarian carcinoma using intact tumor tissue. We selected a small cohort of patients with highly annotated high-grade serous ovarian carcinoma, categorized them by response to neoadjuvant chemotherapy (poor or excellent), and analyzed pre-treatment tumor tissue specimens. Our study uncovered extensive differences in tumor composition between the poor responders and excellent responders to chemotherapy, related to cell cluster organization and localization. This in-depth characterization of high-grade serous ovarian carcinoma tumor tissue from poor and excellent responders showed that spatial interactions between cell clusters may influence chemo-responsiveness more than cluster composition alone.