PMID- 34682795 OWN - NLM STAT- PubMed-not-MEDLINE VI - 10 IP - 20 TI - Defining NASH from a Multi-Omics Systems Biology Perspective. LA - eng PT - Journal Article PT - Review PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 IS - 2077-0383 (Print) LID - 4673 [pii] LID - 10.3390/jcm10204673 [doi] FAU - Niu, Lili AU - Niu L AD - Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark. AD - Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany. FAU - Sulek, Karolina AU - Sulek K AD - Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark. AD - Systems Medicine, Steno Diabetes Center Copenhagen, 2820 Gentofte, Denmark. FAU - Vasilopoulou, Catherine G AU - Vasilopoulou CG AD - Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany. FAU - Santos, Alberto AU - Santos A AD - Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark. AD - Center for Health Data Science, University of Copenhagen, 2200 Copenhagen, Denmark. AD - Big Data Institute, Nuffield Department of Medicine, University of Oxford, Oxford OX3 7LF, UK. FAU - Wewer Albrechtsen, Nicolai J AU - Wewer Albrechtsen NJ AUID- ORCID: 0000-0003-4230-5753 AD - Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark. AD - Department of Clinical Biochemistry, Rigshospitalet, 2100 Copenhagen, Denmark. AD - Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark. FAU - Rasmussen, Simon AU - Rasmussen S AUID- ORCID: 0000-0001-6323-9041 AD - Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark. FAU - Meier, Florian AU - Meier F AD - Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany. AD - Functional Proteomics, Jena University Hospital, 07747 Jena, Germany. FAU - Mann, Matthias AU - Mann M AD - Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark. AD - Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany. IS - 2077-0383 (Linking) OTO - NOTNLM OT - NAFLD OT - biomarker discovery OT - liver disease OT - machine learning OT - multi-omics OT - systems biology PMC - PMC8538576 LR - 20211026 DP - 2021 Oct 12 DEP - 20211012 AB - Non-alcoholic steatohepatitis (NASH) is a chronic liver disease affecting up to 6.5% of the general population. There is no simple definition of NASH, and the molecular mechanism underlying disease pathogenesis remains elusive. Studies applying single omics technologies have enabled a better understanding of the molecular profiles associated with steatosis and hepatic inflammation-the commonly accepted histologic features for diagnosing NASH, as well as the discovery of novel candidate biomarkers. Multi-omics analysis holds great potential to uncover new insights into disease mechanism through integrating multiple layers of molecular information. Despite the technical and computational challenges associated with such efforts, a few pioneering studies have successfully applied multi-omics technologies to investigate NASH. Here, we review the most recent technological developments in mass spectrometry (MS)-based proteomics, metabolomics, and lipidomics. We summarize multi-omics studies and emerging omics biomarkers in NASH and highlight the biological insights gained through these integrated analyses.