PMID- 34572274 OWN - NLM STAT- PubMed-not-MEDLINE VI - 9 IP - 9 TI - Use of MALDI Mass Spectrometry Imaging to Identify Proteomic Signatures in Aortic Aneurysms after Endovascular Repair. LA - eng PT - Journal Article PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 IS - 2227-9059 (Print) LID - 1088 [pii] LID - 10.3390/biomedicines9091088 [doi] FAU - Buerger, Matthias AU - Buerger M AUID- ORCID: 0000-0003-3403-4025 AD - Berlin Institute of Health, Vascular Surgery Clinic, Charité-Universitätsmedizin Berlin, Freie Universität Berlin and Humboldt-Universität zu Berlin, Hindenburgdamm 30, 12203 Berlin, Germany. FAU - Klein, Oliver AU - Klein O AD - BIH Center for Regenerative Therapies BCRT, Berlin Institute of Health at Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany. FAU - Kapahnke, Sebastian AU - Kapahnke S AD - Berlin Institute of Health, Vascular Surgery Clinic, Charité-Universitätsmedizin Berlin, Freie Universität Berlin and Humboldt-Universität zu Berlin, Hindenburgdamm 30, 12203 Berlin, Germany. FAU - Mueller, Verena AU - Mueller V AD - Berlin Institute of Health, Vascular Surgery Clinic, Charité-Universitätsmedizin Berlin, Freie Universität Berlin and Humboldt-Universität zu Berlin, Hindenburgdamm 30, 12203 Berlin, Germany. FAU - Frese, Jan Paul AU - Frese JP AUID- ORCID: 0000-0002-2690-0731 AD - Berlin Institute of Health, Vascular Surgery Clinic, Charité-Universitätsmedizin Berlin, Freie Universität Berlin and Humboldt-Universität zu Berlin, Hindenburgdamm 30, 12203 Berlin, Germany. FAU - Omran, Safwan AU - Omran S AD - Berlin Institute of Health, Vascular Surgery Clinic, Charité-Universitätsmedizin Berlin, Freie Universität Berlin and Humboldt-Universität zu Berlin, Hindenburgdamm 30, 12203 Berlin, Germany. FAU - Greiner, Andreas AU - Greiner A AD - Berlin Institute of Health, Vascular Surgery Clinic, Charité-Universitätsmedizin Berlin, Freie Universität Berlin and Humboldt-Universität zu Berlin, Hindenburgdamm 30, 12203 Berlin, Germany. FAU - Sommerfeld, Manuela AU - Sommerfeld M AD - Center for Cardiovascular Research (CCR), Institute of Pharmacology, Charité-Universitätsmedizin Berlin, Freie Universität Berlin and Humboldt-Universität zu Berlin, Hessische Str. 3-4, 10115 Berlin, Germany. FAU - Kaschina, Elena AU - Kaschina E AD - Center for Cardiovascular Research (CCR), Institute of Pharmacology, Charité-Universitätsmedizin Berlin, Freie Universität Berlin and Humboldt-Universität zu Berlin, Hessische Str. 3-4, 10115 Berlin, Germany. FAU - Jannasch, Anett AU - Jannasch A AUID- ORCID: 0000-0002-8047-2774 AD - Department of Cardiac Surgery, Herzzentrum Dresden, Medical Faculty Carl Gustav Carus Dresden, Technische Universität Dresden, 01307 Dresden, Germany. FAU - Dittfeld, Claudia AU - Dittfeld C AD - Department of Cardiac Surgery, Herzzentrum Dresden, Medical Faculty Carl Gustav Carus Dresden, Technische Universität Dresden, 01307 Dresden, Germany. FAU - Mahlmann, Adrian AU - Mahlmann A AD - University Center for Vascular Medicine, Department of Medicine-Section Angiology, University Hospital Carl Gustav Carus, Technische Universität, 01307 Dresden, Germany. FAU - Hinterseher, Irene AU - Hinterseher I AUID- ORCID: 0000-0001-6725-525X AD - Berlin Institute of Health, Vascular Surgery Clinic, Charité-Universitätsmedizin Berlin, Freie Universität Berlin and Humboldt-Universität zu Berlin, Hindenburgdamm 30, 12203 Berlin, Germany. AD - Medizinische Hochschule Brandenburg Theordor Fontane, 16816 Neuruppin, Germany. IS - 2227-9059 (Linking) OTO - NOTNLM OT - EVAR OT - MALDI-MSI OT - aortic aneurysm OT - endoleak OT - proteomic signature PMC - PMC8465851 LR - 20211001 DP - 2021 Aug 26 DEP - 20210826 AB - Endovascular repair (EVAR) has become the standard procedure in treating thoracic (TAA) or abdominal aortic aneurysms (AAA). Not entirely free of complications, a persisting perfusion of the aneurysm after EVAR, called Endoleak (EL), leads to reintervention and risk of secondary rupture. How the aortic wall responds to the implantation of a stentgraft and EL is mostly uncertain. We present a pilot study to identify peptide signatures and gain new insights in pathophysiological alterations of the aortic wall after EVAR using matrix-assisted laser desorption or ionization mass spectrometry imaging (MALDI-MSI). In course of or accompanying an open aortic repair, tissue sections from 15 patients (TAA = 5, AAA = 5, EVAR = 5) were collected. Regions of interest (tunica media and tunica adventitia) were defined and univariate (receiver operating characteristic analysis) statistical analysis for subgroup comparison was used. This proof-of-concept study demonstrates that MALDI-MSI is feasible to identify discriminatory peptide signatures separating TAA, AAA and EVAR. Decreased intensity distributions for actin, tropomyosin, and troponin after EVAR suggest impaired contractility in vascular smooth muscle cells. Furthermore, inability to provide energy caused by impaired respiratory chain function and continuous degradation of extracellular matrix components (collagen) might support aortic wall destabilization. In case of EL after EVAR, this mechanism may result in a weakened aortic wall with lacking ability to react on reinstating pulsatile blood flow.