PMID- 34393728 OWN - NLM STAT- PubMed-not-MEDLINE VI - 15 TI - Mass Spectrometry Imaging for Glycome in the Brain. PG - 711955 CI - Copyright © 2021 Hasan, Mimi, Mamun, Islam, Waliullah, Nabi, Tamannaa, Kahyo and Setou. LA - eng PT - Journal Article PT - Review PL - Switzerland TA - Front Neuroanat JT - Frontiers in neuroanatomy JID - 101477943 IS - 1662-5129 (Print) LID - 10.3389/fnana.2021.711955 [doi] FAU - Hasan, Md Mahmudul AU - Hasan MM AD - Department of Cellular & Molecular Anatomy, Hamamatsu University School of Medicine, Hamamatsu, Japan. FAU - Mimi, Mst Afsana AU - Mimi MA AD - Department of Cellular & Molecular Anatomy, Hamamatsu University School of Medicine, Hamamatsu, Japan. FAU - Mamun, Md Al AU - Mamun MA AD - Department of Cellular & Molecular Anatomy, Hamamatsu University School of Medicine, Hamamatsu, Japan. FAU - Islam, Ariful AU - Islam A AD - Department of Cellular & Molecular Anatomy, Hamamatsu University School of Medicine, Hamamatsu, Japan. FAU - Waliullah, A S M AU - Waliullah ASM AD - Department of Cellular & Molecular Anatomy, Hamamatsu University School of Medicine, Hamamatsu, Japan. FAU - Nabi, Md Mahamodun AU - Nabi MM AD - Department of Cellular & Molecular Anatomy, Hamamatsu University School of Medicine, Hamamatsu, Japan. FAU - Tamannaa, Zinat AU - Tamannaa Z AD - Department of Cellular & Molecular Anatomy, Hamamatsu University School of Medicine, Hamamatsu, Japan. FAU - Kahyo, Tomoaki AU - Kahyo T AD - Department of Cellular & Molecular Anatomy, Hamamatsu University School of Medicine, Hamamatsu, Japan. AD - International Mass Imaging Center, Hamamatsu University School of Medicine, Hamamatsu, Japan. FAU - Setou, Mitsutoshi AU - Setou M AD - Department of Cellular & Molecular Anatomy, Hamamatsu University School of Medicine, Hamamatsu, Japan. AD - International Mass Imaging Center, Hamamatsu University School of Medicine, Hamamatsu, Japan. AD - Department of Systems Molecular Anatomy, Institute for Medical Photonics Research, Preeminent Medical Photonics Education & Research Center, Hamamatsu, Japan. IS - 1662-5129 (Linking) OTO - NOTNLM OT - brain tissue OT - glycans OT - glycosylation OT - mass spectrometry imaging OT - three-dimensional MSI PMC - PMC8358800 LR - 20210817 DP - 2021 DEP - 20210729 AB - Glycans are diverse structured biomolecules that play crucial roles in various biological processes. Glycosylation, an enzymatic system through which various glycans are bound to proteins and lipids, is the most common and functionally crucial post-translational modification process. It is known to be associated with brain development, signal transduction, molecular trafficking, neurodegenerative disorders, psychopathologies, and brain cancers. Glycans in glycoproteins and glycolipids expressed in brain cells are involved in neuronal development, biological processes, and central nervous system maintenance. The composition and expression of glycans are known to change during those physiological processes. Therefore, imaging of glycans and the glycoconjugates in the brain regions has become a "hot" topic nowadays. Imaging techniques using lectins, antibodies, and chemical reporters are traditionally used for glycan detection. However, those techniques offer limited glycome detection. Mass spectrometry imaging (MSI) is an evolving field that combines mass spectrometry with histology allowing spatial and label-free visualization of molecules in the brain. In the last decades, several studies have employed MSI for glycome imaging in brain tissues. The current state of MSI uses on-tissue enzymatic digestion or chemical reaction to facilitate successful glycome imaging. Here, we reviewed the available literature that applied MSI techniques for glycome visualization and characterization in the brain. We also described the general methodologies for glycome MSI and discussed its potential use in the three-dimensional MSI in the brain.