PMID- 34028638 OWN - NLM STAT- In-Process VI - 79 IP - 4 TI - Discovery of Plasma Membrane-Associated RNAs through APEX-seq. PG - 905-917 CI - © 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. LA - eng PT - Journal Article PL - United States TA - Cell Biochem Biophys JT - Cell biochemistry and biophysics JID - 9701934 IS - 1559-0283 (Electronic) LID - 10.1007/s12013-021-00991-0 [doi] FAU - Wu, Erzhong AU - Wu E AD - Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, 100101, Beijing, China. AD - University of Chinese Academy of Sciences, 100049, Beijing, China. FAU - Guo, Xuzhen AU - Guo X AD - Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, 100101, Beijing, China. AD - University of Chinese Academy of Sciences, 100049, Beijing, China. FAU - Teng, Xueyi AU - Teng X AD - Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, 100101, Beijing, China. AD - University of Chinese Academy of Sciences, 100049, Beijing, China. FAU - Zhang, Ruijin AU - Zhang R AD - Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, 100101, Beijing, China. AD - University of Chinese Academy of Sciences, 100049, Beijing, China. FAU - Li, Fahui AU - Li F AD - Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, 100101, Beijing, China. FAU - Cui, Ya AU - Cui Y AD - Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, 100101, Beijing, China. FAU - Zhang, Dongdong AU - Zhang D AD - Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, 100101, Beijing, China. FAU - Liu, Qinghua AU - Liu Q AD - Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, 100101, Beijing, China. FAU - Luo, Jianjun AU - Luo J AD - Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, 100101, Beijing, China. luojianj@ibp.ac.cn. AD - University of Chinese Academy of Sciences, 100049, Beijing, China. luojianj@ibp.ac.cn. FAU - Wang, Jiangyun AU - Wang J AD - Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, 100101, Beijing, China. jwang@ibp.ac.cn. AD - University of Chinese Academy of Sciences, 100049, Beijing, China. jwang@ibp.ac.cn. FAU - Chen, Runsheng AU - Chen R AUID- ORCID: http://orcid.org/0000-0001-6049-8347 AD - Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, 100101, Beijing, China. rschen@ibp.ac.cn. AD - University of Chinese Academy of Sciences, 100049, Beijing, China. rschen@ibp.ac.cn. IS - 1085-9195 (Linking) SB - IM OTO - NOTNLM OT - APEX-seq OT - LncRNA OT - PMAR72 OT - Plasma membrane LR - 20211101 DP - 2021 Dec DEP - 20210524 AB - In addition to nucleic acids, a variety of other biomolecules have also been found on the plasma membrane. Although researchers have realized that RNA has the ability to bind to membrane vesicles in vitro, little is known about whether and how RNA connects to the plasma membrane of the cell. The combination of high-throughput sequencing and in situ labeling methods provides an innovative approach for large-scale identification of subcellular RNAs. Here, we applied the recently published method APEX-seq and identified 75 RNAs related to the plasma membrane, in which lncRNA PMAR72 (plasma membrane-associated RNA AL121772.1) has a considerable affinity with sphingomyelin (SM) and localizes within distinct membrane foci. Our findings will provide some new evidence to elaborate the relationship between RNA and the plasma membrane of mammalian cells.