PMID- 34008302 OWN - NLM STAT- MEDLINE VI - 17 IP - 24 TI - Noncontact Multiphysics Probe for Spatiotemporal Resolved Single-Cell Manipulation and Analyses. PG - e2100801 CI - © 2021 The Authors. Small published by Wiley-VCH GmbH. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Germany TA - Small JT - Small (Weinheim an der Bergstrasse, Germany) JID - 101235338 IS - 1613-6829 (Electronic) LID - 10.1002/smll.202100801 [doi] FAU - Brimmo, Ayoola T AU - Brimmo AT AD - Division of Engineering, New York University Abu Dhabi (NYUAD), P.O. Box 129188, Abu Dhabi, UAE. AD - Department of Mechanical and Aerospace Engineering, New York University, Brooklyn, NY, 11201, USA. FAU - Menachery, Anoop AU - Menachery A AD - Division of Engineering, New York University Abu Dhabi (NYUAD), P.O. Box 129188, Abu Dhabi, UAE. FAU - Sukumar, Pavithra AU - Sukumar P AD - Division of Engineering, New York University Abu Dhabi (NYUAD), P.O. Box 129188, Abu Dhabi, UAE. FAU - Qasaimeh, Mohammad A AU - Qasaimeh MA AUID- ORCID: 0000-0003-1122-9698 AD - Division of Engineering, New York University Abu Dhabi (NYUAD), P.O. Box 129188, Abu Dhabi, UAE. AD - Department of Mechanical and Aerospace Engineering, New York University, Brooklyn, NY, 11201, USA. IS - 1613-6810 (Linking) SB - IM MH - *Microfluidics MH - *Single-Cell Analysis OTO - NOTNLM OT - *biopsy OT - *electropermealization OT - *microfluidic probe OT - *singe cell OT - *tissue culture OT - *transcriptomics OT - *transfection OT - *tweezing DCOM- 20210714 LR - 20210714 DP - 202106 DEP - 20210519 AB - Heterogeneity and spatial arrangement of individual cells within tissues are critical to the identity of the host multicellular organism. While current single-cell techniques are capable of resolving heterogeneity, they mostly rely on extracting target cells from their physiological environment and hence lose the spatiotemporal resolution required for understanding cellular networks. Here, a multifunctional noncontact scanning probe that can precisely perform multiple manipulation procedures on living single-cells, while within their physiological tissue environment, is demonstrated. The noncontact multiphysics probe (NMP) consists of fluidic apertures and "hump" shaped electrodes that simultaneously confine reagents and electric signals with a single-cell resolution. The NMP's unique electropermealization-based approach in transferring macromolecules through the cell membrane is presented. The technology's adjustable spatial ability is demonstrated by transfecting adjacent single-cells with different DNA plasmid vectors. The NMP technology also opens the door for controllable cytoplasm extraction from living single-cells. This powerful application is demonstrated by executing multiple time point biopsies on adherent cells without affecting the integrity of the extracted macromolecules or the viability of cells. Furthermore, the NMP's function as an electro-thermal based microfluidic whole-cell tweezer is reported. This work offers a multifunctional tool with unprecedented probing features for spatiotemporal single-cell analysis within tissue samples.