PMID- 33629280 OWN - NLM STAT- MEDLINE VI - 18 IP - 2 TI - Single-Cell RNA Sequencing of the Adult Mammalian Heart-State-of-the-Art and Future Perspectives. PG - 64-70 LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Review PL - United States TA - Curr Heart Fail Rep JT - Current heart failure reports JID - 101196487 IS - 1546-9549 (Electronic) LID - 10.1007/s11897-021-00504-3 [doi] FAU - Gladka, Monika M AU - Gladka MM AUID- ORCID: http://orcid.org/0000-0002-4094-4335 AD - Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Centre, PO Box 85164, 3508 AD, Utrecht, The Netherlands. m.gladka@hubrecht.eu. AD - Department of Medical Biology, Amsterdam University Medical Center, Amsterdam, The Netherlands. m.gladka@hubrecht.eu. IS - 1546-9530 (Linking) SB - IM MH - Adult MH - Animals MH - Base Sequence MH - Heart MH - *Heart Failure/genetics MH - Humans MH - Mice MH - Sequence Analysis, RNA MH - *Single-Cell Analysis OTO - NOTNLM OT - *Cardiac disease OT - *Cardiomyocytes OT - *Heart OT - *Single-cell RNA sequencing PMC - PMC7954708 DCOM- 20211025 LR - 20211025 DP - 202104 DEP - 20210225 AB - PURPOSE OF THE REVIEW: Cardiovascular disease remains the leading cause of death worldwide, resulting in cardiac dysfunction and, subsequently, heart failure (HF). Single-cell RNA sequencing (scRNA-seq) is a rapidly developing tool for studying the transcriptional heterogeneity in both healthy and diseased hearts. Wide applications of techniques like scRNA-seq could significantly contribute to uncovering the molecular mechanisms involved in the onset and progression to HF and contribute to the development of new, improved therapies. This review discusses several studies that successfully applied scRNA-seq to the mouse and human heart using various methods of tissue processing and downstream analysis. RECENT FINDINGS: The application of scRNA-seq in the cardiovascular field is continuously expanding, providing new detailed insights into cardiac pathophysiology. Increased understanding of cardiac pathophysiology on the single-cell level will contribute to the development of novel, more effective therapeutic strategies. Here, we summarise the possible application of scRNA-seq to the adult mammalian heart.