PMID- 33620075 OWN - NLM STAT- MEDLINE VI - 5 IP - 2 TI - Mapping the plant proteome: tools for surveying coordinating pathways. PG - 203-220 CI - © 2021 The Author(s). LA - eng PT - Journal Article PT - Research Support, U.S. Gov't, Non-P.H.S. PT - Review PL - England TA - Emerg Top Life Sci JT - Emerging topics in life sciences JID - 101706399 IS - 2397-8554 (Print) LID - 10.1042/ETLS20200270 [doi] FAU - Smythers, Amanda L AU - Smythers AL AUID- ORCID: 0000-0002-1552-6705 AD - Department of Chemistry, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, U.S.A. FAU - Hicks, Leslie M AU - Hicks LM AUID- ORCID: 0000-0002-8008-3998 AD - Department of Chemistry, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, U.S.A. IS - 2397-8554 (Linking) RN - 0 (Proteome) SB - IM MH - Mass Spectrometry MH - Plants/metabolism MH - Protein Processing, Post-Translational MH - *Proteome/genetics/metabolism MH - *Proteomics OTO - NOTNLM OT - plant proteins OT - post translational modification OT - protein–protein interactions OT - proteomics PMC - PMC8166341 DCOM- 20211022 LR - 20231111 DP - 20210521 AB - Plants rapidly respond to environmental fluctuations through coordinated, multi-scalar regulation, enabling complex reactions despite their inherently sessile nature. In particular, protein post-translational signaling and protein-protein interactions combine to manipulate cellular responses and regulate plant homeostasis with precise temporal and spatial control. Understanding these proteomic networks are essential to addressing ongoing global crises, including those of food security, rising global temperatures, and the need for renewable materials and fuels. Technological advances in mass spectrometry-based proteomics are enabling investigations of unprecedented depth, and are increasingly being optimized for and applied to plant systems. This review highlights recent advances in plant proteomics, with an emphasis on spatially and temporally resolved analysis of post-translational modifications and protein interactions. It also details the necessity for generation of a comprehensive plant cell atlas while highlighting recent accomplishments within the field.