PMID- 33513863 OWN - NLM STAT- MEDLINE VI - 22 IP - 3 TI - Integrating LCM-Based Spatio-Temporal Transcriptomics Uncovers Conceptus and Endometrial Luminal Epithelium Communication that Coordinates the Conceptus Attachment in Pigs. LA - eng PT - Journal Article PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 IS - 1422-0067 (Electronic) LID - 1248 [pii] LID - 10.3390/ijms22031248 [doi] FAU - Wang, Feiyu AU - Wang F AD - Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. FAU - Zhao, Shilei AU - Zhao S AD - Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. FAU - Deng, Dadong AU - Deng D AD - Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. FAU - Wang, Weiwei AU - Wang W AD - Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. FAU - Xu, Xuewen AU - Xu X AUID- ORCID: 0000-0001-6925-3346 AD - Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. FAU - Liu, Xiaolei AU - Liu X AD - Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. FAU - Zhao, Shuhong AU - Zhao S AD - Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. FAU - Yu, Mei AU - Yu M AD - Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. IS - 1422-0067 (Linking) SB - IM MH - Animals MH - Cell Communication/*genetics MH - Embryo Implantation/genetics MH - Embryo, Mammalian MH - Endometrium/growth & development/*metabolism MH - Epithelium/metabolism MH - Female MH - Laser Capture Microdissection MH - Pregnancy MH - Swine/*genetics/growth & development MH - Transcriptome/*genetics MH - Uterus/growth & development/metabolism OTO - NOTNLM OT - conceptus attachment OT - endometrial luminal epithelium OT - laser capture microdissection OT - pigs OT - spatio-temporal transcriptomics PMC - PMC7866100 DCOM- 20210908 LR - 20210908 DP - 2021 Jan 27 DEP - 20210127 AB - Attachment of conceptus to the endometrial luminal epithelium (LE) is a critical event for early placentation in Eutheria. Since the attachment occurs at a particular site within the uterus, a coordinated communication between three spatially distinct compartments (conceptus and endometrial LE from two anatomical regions of the uterus to which conceptus attaches and does not attach) is essential but remains to be fully characterized. Using the laser capture microdissection (LCM) technique, we firstly developed an approach that can allow us to pair the pig conceptus sample with its nearby endometrial epithelium sample without losing the native spatial information. Then, a comprehensive spatio-temporal transcriptomic profile without losing the original conceptus-endometrium coordinates was constructed. The analysis shows that an apparent difference in transcriptional responses to the conceptus exists between the endometrial LE from the two anatomically distinct regions in the uterus. In addition, we identified the communication pathways that link the conceptus and endometrial LE and found that these pathways have important roles in conceptus attachment. Furthermore, a number of genes whose expression is spatially restricted in the two different anatomical regions within the uterus were characterized for the first time and two of them (SULT2A1 and MEP1B) may cooperatively contribute to establish conceptus attachment in pigs. The results from our study have implications in understanding of conceptus/embryo attachment in pigs and other large polytocous species.