PMID- 33367236 OWN - NLM STAT- MEDLINE VI - 1 IP - 3 TI - Integrative Multi-omics Analyses of Barley Rootzones under Salinity Stress Reveal Two Distinctive Salt Tolerance Mechanisms. PG - 100031 CI - © 2020 The Author(s). LA - eng PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - China TA - Plant Commun JT - Plant communications JID - 101769147 IS - 2590-3462 (Electronic) LID - 10.1016/j.xplc.2020.100031 [doi] FAU - Ho, William Wing Ho AU - Ho WWH AD - School of BioSciences, The University of Melbourne, Parkville, VIC 3010, Australia. FAU - Hill, Camilla B AU - Hill CB AD - School of BioSciences, The University of Melbourne, Parkville, VIC 3010, Australia. AD - School of Veterinary and Life Sciences, Murdoch University, Murdoch, WA 6150, Australia. FAU - Doblin, Monika S AU - Doblin MS AD - La Trobe Institute for Agriculture & Food, Department of Animal, Plant and Soil Science, La Trobe University, Bundoora, VIC 3086, Australia. FAU - Shelden, Megan C AU - Shelden MC AD - ARC Centre of Excellence in Plant Energy Biology, School of Agriculture, Food and Wine, University of Adelaide, Glen Osmond, SA 5064, Australia. FAU - van de Meene, Allison AU - van de Meene A AD - School of BioSciences, The University of Melbourne, Parkville, VIC 3010, Australia. FAU - Rupasinghe, Thusitha AU - Rupasinghe T AD - School of BioSciences, The University of Melbourne, Parkville, VIC 3010, Australia. FAU - Bacic, Antony AU - Bacic A AD - La Trobe Institute for Agriculture & Food, Department of Animal, Plant and Soil Science, La Trobe University, Bundoora, VIC 3086, Australia. FAU - Roessner, Ute AU - Roessner U AD - School of BioSciences, The University of Melbourne, Parkville, VIC 3010, Australia. AD - Metabolomics Australia, The University of Melbourne, Parkville, VIC 3010, Australia. IS - 2590-3462 (Linking) MH - Africa, Northern MH - Gene Expression Profiling MH - Genotype MH - Hordeum/*genetics/*physiology MH - Lipidomics MH - Metabolome/drug effects MH - Plant Roots/genetics/physiology MH - Salt Stress/*genetics/*physiology MH - Salt Tolerance/*genetics/*physiology MH - Transcriptome/drug effects OTO - NOTNLM OT - *barley root OT - *lipidomics OT - *metabolomics OT - *omics integration OT - *salinity stress OT - *transcriptomics PMC - PMC7748018 DCOM- 20211101 LR - 20211101 DP - 20200511 DEP - 20200213 AB - The mechanisms underlying rootzone-localized responses to salinity during early stages of barley development remain elusive. In this study, we performed the analyses of multi-root-omes (transcriptomes, metabolomes, and lipidomes) of a domesticated barley cultivar (Clipper) and a landrace (Sahara) that maintain and restrict seedling root growth under salt stress, respectively. Novel generalized linear models were designed to determine differentially expressed genes (DEGs) and abundant metabolites (DAMs) specific to salt treatments, genotypes, or rootzones (meristematic Z1, elongation Z2, and maturation Z3). Based on pathway over-representation of the DEGs and DAMs, phenylpropanoid biosynthesis is the most statistically enriched biological pathway among all salinity responses observed. Together with histological evidence, an intense salt-induced lignin impregnation was found only at stelic cell wall of Clipper Z2, compared with a unique elevation of suberin deposition across Sahara Z2. This suggests two differential salt-induced modulations of apoplastic flow between the genotypes. Based on the global correlation network of the DEGs and DAMs, callose deposition that potentially adjusted symplastic flow in roots was almost independent of salinity in rootzones of Clipper, and was markedly decreased in Sahara. Taken together, we propose two distinctive salt tolerance mechanisms in Clipper (growth-sustaining) and Sahara (salt-shielding), providing important clues for improving crop plasticity to cope with deteriorating global soil salinization.