PMID- 31954659 OWN - NLM STAT- MEDLINE VI - 10 IP - 1 TI - Cellular Dialogues: Cell-Cell Communication through Diffusible Molecules Yields Dynamic Spatial Patterns. PG - 82-98.e7 CI - Copyright © 2019 The Author(s). Published by Elsevier Inc. All rights reserved. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Cell Syst JT - Cell systems JID - 101656080 IS - 2405-4720 (Electronic) LID - S2405-4712(19)30431-4 [pii] LID - 10.1016/j.cels.2019.12.001 [doi] FAU - Dang, Yiteng AU - Dang Y AD - Kavli Institute of Nanoscience, Delft University of Technology, Delft 2629HZ, the Netherlands; Department of Bionanoscience, Delft University of Technology, Delft 2629HZ, the Netherlands. FAU - Grundel, Douwe A J AU - Grundel DAJ AD - Kavli Institute of Nanoscience, Delft University of Technology, Delft 2629HZ, the Netherlands; Department of Bionanoscience, Delft University of Technology, Delft 2629HZ, the Netherlands. FAU - Youk, Hyun AU - Youk H AD - Kavli Institute of Nanoscience, Delft University of Technology, Delft 2629HZ, the Netherlands; Department of Bionanoscience, Delft University of Technology, Delft 2629HZ, the Netherlands; CIFAR, CIFAR Azrieli Global Scholars Program, Toronto, ON M5G 1M1, Canada. Electronic address: h.youk@tudelft.nl. IS - 2405-4712 (Linking) SB - IM MH - Cell Communication/*genetics MH - Gene Regulatory Networks/*genetics MH - Humans MH - Models, Biological OTO - NOTNLM OT - cell-cell communication OT - cellular automata OT - complex systems OT - gene networks OT - multicellular systems OT - pattern formation OT - reaction-diffusion OT - self organization OT - spatial patterns OT - waves PMC - PMC6975168 DCOM- 20210608 LR - 20230120 DP - 20200122 DEP - 20200115 AB - Cells form spatial patterns by coordinating their gene expressions. How a group of mesoscopic numbers (hundreds to thousands) of cells, without pre-existing morphogen gradients and spatial organization, self-organizes spatial patterns remains poorly understood. Of particular importance are dynamic spatial patterns such as spiral waves that perpetually move and transmit information. We developed an open-source software for simulating a field of cells that communicate by secreting any number of molecules. With this software and a theory, we identified all possible "cellular dialogues"-ways of communicating with two diffusing molecules-that yield diverse dynamic spatial patterns. These patterns emerge despite widely varying responses of cells to the molecules, gene-expression noise, spatial arrangements, and cell movements. A three-stage, "order-fluctuate-settle" process forms dynamic spatial patterns: cells form long-lived whirlpools of wavelets that, following erratic dynamics, settle into a dynamic spatial pattern. Our work helps in identifying gene-regulatory networks that underlie dynamic pattern formations.