PMID- 30644093 OWN - NLM STAT- MEDLINE VI - 234 IP - 8 TI - Region-resolved proteomics profiling of monkey heart. PG - 13720-13734 CI - © 2019 Wiley Periodicals, Inc. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - J Cell Physiol JT - Journal of cellular physiology JID - 0050222 IS - 1097-4652 (Electronic) LID - 10.1002/jcp.28052 [doi] FAU - Hu, Hao-Liang AU - Hu HL AUID- ORCID: 0000-0003-4936-3411 AD - The National & Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan Normal University, Changsha, China. FAU - Kang, Yu AU - Kang Y AD - Division of Cardiology, West China Hospital, Sichuan University, Chengdu, Sichuan, China. FAU - Zeng, Yong AU - Zeng Y AD - The National & Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan Normal University, Changsha, China. FAU - Zhang, Ming AU - Zhang M AD - Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences &Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences (CAS), Kunming, Yunnan, China. FAU - Liao, Qiong AU - Liao Q AD - The National & Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan Normal University, Changsha, China. FAU - Rong, Ming-Qiang AU - Rong MQ AUID- ORCID: 0000-0002-4865-1035 AD - The National & Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan Normal University, Changsha, China. FAU - Zhang, Qin AU - Zhang Q AD - Division of Cardiology, West China Hospital, Sichuan University, Chengdu, Sichuan, China. FAU - Lai, Ren AU - Lai R AD - Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences &Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences (CAS), Kunming, Yunnan, China. IS - 0021-9541 (Linking) RN - 0 (Proteome) SB - IM MH - Animals MH - *Cardiovascular System MH - Computational Biology MH - *Heart MH - *Heart Valves MH - Macaca mulatta MH - Male MH - *Myocardium MH - Proteome MH - Proteomics MH - Reference Values OTO - NOTNLM OT - *bioinformatics analysis OT - *gene-disease associations OT - *monkey heart OT - *proteomics PMC - PMC7166496 DCOM- 20200527 LR - 20200527 DP - 201908 DEP - 20190115 AB - Nonhuman primates (NHPs) play an indispensable role in biomedical research because of their similarities in genetics, physiological, and neurological function to humans. Proteomics profiling of monkey heart could reveal significant cardiac biomarkers and help us to gain a better understanding of the pathogenesis of heart disease. However, the proteomic study of monkey heart is relatively lacking. Here, we performed the proteomics profiling of the normal monkey heart by measuring three major anatomical regions (vessels, valves, and chambers) based on iTRAQ-coupled LC-MS/MS analysis. Over 3,200 proteins were identified and quantified from three heart tissue samples. Furthermore, multiple bioinformatics analyses such as gene ontology analysis, protein-protein interaction analysis, and gene-diseases association were used to investigate biological network of those proteins from each area. More than 60 genes in three heart regions are implicated with heart diseases such as hypertrophic cardiomyopathy, heart failure, and myocardial infarction. These genes associated with heart disease are mainly enriched in citrate cycle, amino acid degradation, and glycolysis pathway. At the anatomical level, the revelation of molecular characteristics of the healthy monkey heart would be an important starting point to investigate heart disease. As a unique resource, this study can serve as a reference map for future in-depth research on cardiac disease-related NHP model and novel biomarkers of cardiac injury.