PMID- 30250804 OWN - NLM STAT- PubMed-not-MEDLINE VI - 5 IP - 9 TI - Highly Multiplexed Single-Cell Protein Profiling with Large-Scale Convertible DNA-Antibody Barcoded Arrays. PG - 1800672 LA - eng PT - Journal Article PL - Germany TA - Adv Sci (weinh) JT - Advanced science (Weinheim, Baden-Wurttemberg, Germany) JID - 101664569 IS - 2198-3844 (Print) LID - 10.1002/advs.201800672 [doi] FAU - Zhao, Peng AU - Zhao P AD - Multiplex Biotechnology Laboratory Department of Chemistry University at Albany State University of New York Albany NY 12222 USA. FAU - Bhowmick, Sirsendu AU - Bhowmick S AD - Multiplex Biotechnology Laboratory Department of Chemistry University at Albany State University of New York Albany NY 12222 USA. FAU - Yu, Jianchao AU - Yu J AD - Multiplex Biotechnology Laboratory Department of Chemistry University at Albany State University of New York Albany NY 12222 USA. FAU - Wang, Jun AU - Wang J AUID- ORCID: 0000-0002-8781-8248 AD - Multiplex Biotechnology Laboratory Department of Chemistry University at Albany State University of New York Albany NY 12222 USA. AD - Cancer Research Center University at Albany State University of New York Rensselaer NY 12144 USA. IS - 2198-3844 (Linking) OTO - NOTNLM OT - DNA encoding OT - barcoded arrays OT - multiplex OT - protein detection OT - singleā€cell measurements PMC - PMC6145231 LR - 20230928 DP - 2018 Sep DEP - 20180802 AB - Highly multiplexed detection of proteins secreted by single cells is always challenging. Herein, a multiplexed in situ tagging technique based on single-stranded DNA encoded microbead arrays and multicolor successive imaging for assaying single-cell secreted proteins with high throughput and high sensitivity is presented. This technology is demonstrated to be capable of increasing the multiplexity exponentially. Upon integration with polydimethylsiloxane microwells, this platform is applied to detect ten immune effector proteins from differentiated single macrophages stimulated with lipopolysaccharide. Significant heterogeneity is observed when the derived human primary macrophages are analyzed. This versatile technology is expected to open new opportunities in systems biology, immune regulation studies, signaling analysis, and molecular diagnostics.