PMID- 29804301 OWN - NLM STAT- MEDLINE VI - 146 IP - 5 TI - Subventricular zone lipidomic architecture loss in Huntington's disease. PG - 613-630 CI - © 2018 International Society for Neurochemistry. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - J Neurochem JT - Journal of neurochemistry JID - 2985190R IS - 1471-4159 (Electronic) LID - 10.1111/jnc.14468 [doi] FAU - Hunter, Mandana AU - Hunter M AD - Department of Pharmacology and Clinical Pharmacology, University of Auckland, Auckland, New Zealand. AD - Centre for Brain Research, University of Auckland, Auckland, New Zealand. FAU - Demarais, Nicholas J AU - Demarais NJ AD - School of Biological Sciences, University of Auckland, Auckland, New Zealand. FAU - Faull, Richard L M AU - Faull RLM AD - Centre for Brain Research, University of Auckland, Auckland, New Zealand. AD - Department of Anatomy and Medical Imaging, University of Auckland, Auckland, New Zealand. FAU - Grey, Angus C AU - Grey AC AD - Centre for Brain Research, University of Auckland, Auckland, New Zealand. AD - Department of Physiology, University of Auckland, Auckland, New Zealand. FAU - Curtis, Maurice A AU - Curtis MA AUID- ORCID: 0000-0003-4496-0233 AD - Centre for Brain Research, University of Auckland, Auckland, New Zealand. AD - Department of Anatomy and Medical Imaging, University of Auckland, Auckland, New Zealand. IS - 0022-3042 (Linking) RN - 0 (Lipids) RN - 0 (Sphingomyelins) RN - 0 (Sulfoglycosphingolipids) RN - 0 (Triglycerides) RN - EC 3.1.3.- (ceramide-1-phosphate phosphatase) RN - EC 3.1.3.2 (Phosphoric Monoester Hydrolases) SB - IM MH - Adult MH - Aged MH - Autopsy MH - Female MH - Fourier Analysis MH - Humans MH - Huntington Disease/*pathology MH - Lateral Ventricles/*metabolism/*pathology MH - *Lipid Metabolism MH - Lipids MH - Male MH - Mass Spectrometry MH - Middle Aged MH - Phosphoric Monoester Hydrolases MH - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization MH - Sphingomyelins MH - Sulfoglycosphingolipids MH - Triglycerides OTO - NOTNLM OT - * MALDI OT - *Huntington's disease OT - *imaging mass spectrometry OT - *lipids OT - *neurogenesis OT - *subventricular zone DCOM- 20190626 LR - 20190626 DP - 201809 DEP - 20180808 AB - The human subventricular zone (SVZ) has a defined cytological and neurochemical architecture, with four constituent laminae that act in concert to support its neurogenic activity. Lipidomic specialisation has previously been demonstrated in the neurologically normal human SVZ, with enrichment of functionally important lipid classes in each lamina. The SVZ is also responsive to neurodegenerative disorders, where thickening of the niche and enhanced proliferation of resident cells were observed in Huntington's disease (HD) brains. In this study, we hypothesised lipidomic changes in the HD SVZ. Using matrix-assisted laser desorption/ionisation (MALDI) imaging mass spectrometry, this analysis shows differences in the lipidomic architecture in the post-mortem Vonsattel grade III cases. Relative to matched, neurologically normal specimens (N = 4), the lipidomic signature of the HD SVZ (N = 4) was characterized by loss of sulfatides and triglycerides in the myelin layer, with an ectopic and focal accumulation of sphingomyelins and ceramide-1-phosphate observed in this lamina. A striking loss of lipidomic patterning was also observed in the ependymal layer, where the local abundance of phosphatidylinositols was significantly reduced in HD. This comprehensive spatially resolved lipidomic analysis of the human HD SVZ identifies alterations in lipid architecture that may shed light on the mechanisms of SVZ responses to neurodegeneration in HD. Open Science: This manuscript was awarded with the Open Materials Badge. For more information see: https://cos.io/our-services/open-science-badges/.