PMID- 29641186 OWN - NLM STAT- MEDLINE VI - 90 IP - 9 TI - Spatial-Resolution Cell Type Proteome Profiling of Cancer Tissue by Fully Integrated Proteomics Technology. PG - 5879-5886 LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Anal Chem JT - Analytical chemistry JID - 0370536 IS - 1520-6882 (Electronic) LID - 10.1021/acs.analchem.8b00596 [doi] FAU - Xu, Ruilian AU - Xu R AD - Shenzhen People's Hospital , The Second Clinical Medical College of Jinan University , Shenzhen 518020 , China. FAU - Tang, Jun AU - Tang J AD - Shenzhen People's Hospital , The Second Clinical Medical College of Jinan University , Shenzhen 518020 , China. AD - Department of Chemistry , Southern University of Science and Technology , Shenzhen 518055 , China. FAU - Deng, Quantong AU - Deng Q AD - Shenzhen People's Hospital , The Second Clinical Medical College of Jinan University , Shenzhen 518020 , China. FAU - He, Wan AU - He W AD - Shenzhen People's Hospital , The Second Clinical Medical College of Jinan University , Shenzhen 518020 , China. FAU - Sun, Xiujie AU - Sun X AD - Department of Chemistry , Southern University of Science and Technology , Shenzhen 518055 , China. FAU - Xia, Ligang AU - Xia L AD - Shenzhen People's Hospital , The Second Clinical Medical College of Jinan University , Shenzhen 518020 , China. FAU - Cheng, Zhiqiang AU - Cheng Z AD - Shenzhen People's Hospital , The Second Clinical Medical College of Jinan University , Shenzhen 518020 , China. FAU - He, Lisheng AU - He L AD - Shenzhen People's Hospital , The Second Clinical Medical College of Jinan University , Shenzhen 518020 , China. FAU - You, Shuyuan AU - You S AD - Shenzhen People's Hospital , The Second Clinical Medical College of Jinan University , Shenzhen 518020 , China. FAU - Hu, Jintao AU - Hu J AD - Shenzhen People's Hospital , The Second Clinical Medical College of Jinan University , Shenzhen 518020 , China. FAU - Fu, Yuxiang AU - Fu Y AD - Shenzhen People's Hospital , The Second Clinical Medical College of Jinan University , Shenzhen 518020 , China. FAU - Zhu, Jian AU - Zhu J AD - Shenzhen People's Hospital , The Second Clinical Medical College of Jinan University , Shenzhen 518020 , China. FAU - Chen, Yixin AU - Chen Y AD - Shenzhen People's Hospital , The Second Clinical Medical College of Jinan University , Shenzhen 518020 , China. FAU - Gao, Weina AU - Gao W AD - Department of Chemistry , Southern University of Science and Technology , Shenzhen 518055 , China. FAU - He, An AU - He A AD - Department of Chemistry , Southern University of Science and Technology , Shenzhen 518055 , China. FAU - Guo, Zhengyu AU - Guo Z AD - Department of Chemistry , Southern University of Science and Technology , Shenzhen 518055 , China. FAU - Lin, Lin AU - Lin L AD - Department of Chemistry , Southern University of Science and Technology , Shenzhen 518055 , China. FAU - Li, Hua AU - Li H AD - Department of Chemistry , Southern University of Science and Technology , Shenzhen 518055 , China. FAU - Hu, Chaofeng AU - Hu C FAU - Tian, Ruijun AU - Tian R AUID- ORCID: 0000-0001-9521-5124 AD - Department of Chemistry , Southern University of Science and Technology , Shenzhen 518055 , China. AD - Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research , Shenzhen 518055 , China. IS - 0003-2700 (Linking) RN - 0 (Proteome) SB - IM MH - Colonic Neoplasms/*chemistry/pathology MH - Humans MH - Proteome/*analysis MH - *Proteomics DCOM- 20190423 LR - 20190423 DP - 20180501 DEP - 20180418 AB - Increasing attention has been focused on cell type proteome profiling for understanding the heterogeneous multicellular microenvironment in tissue samples. However, current cell type proteome profiling methods need large amounts of starting materials which preclude their application to clinical tumor specimens with limited access. Here, by seamlessly combining laser capture microdissection and integrated proteomics sample preparation technology SISPROT, specific cell types in tumor samples could be precisely dissected with single cell resolution and processed for high-sensitivity proteome profiling. Sample loss and contamination due to the multiple transfer steps are significantly reduced by the full integration and noncontact design. H&E staining dyes which are necessary for cell type investigation could be selectively removed by the unique two-stage design of the spintip device. This easy-to-use proteome profiling technology achieved high sensitivity with the identification of more than 500 proteins from only 0.1 mm2 and 10 μm thickness colon cancer tissue section. The first cell type proteome profiling of four cell types from one colon tumor and surrounding normal tissue, including cancer cells, enterocytes, lymphocytes, and smooth muscle cells, was obtained. 5271, 4691, 4876, and 2140 protein groups were identified, respectively, from tissue section of only 5 mm2 and 10 μm thickness. Furthermore, spatially resolved proteome distribution profiles of enterocytes, lymphocytes, and smooth muscle cells on the same tissue slices and across four consecutive sections with micrometer distance were successfully achieved. This fully integrated proteomics technology, termed LCM-SISPROT, is therefore promising for spatial-resolution cell type proteome profiling of tumor microenvironment with a minute amount of clinical starting materials.